International Crop Research Institute for Semi-arid Tropics (ICRISAT), Patancheru 502 324, Telangana State, India.
Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, 201 313, India.
Bioresour Technol. 2020 Jul;307:123245. doi: 10.1016/j.biortech.2020.123245. Epub 2020 Mar 24.
Diatom algae can produce bioactive compounds like fucoxanthin (FX) and ecosapentaenoic acid (EPA) which are of high demand in pharmaceutical and nutraceutical industries. Here, the influence of different light regimes in combination with major nutrients on growth, FX and EPA production by marine diatom Thalassiosira weissflogii were investigated. Batch cultures of T. weissflogii were illuminated under blue (BL), red (RL) and white (WL) light at two intensities. BL regime resulted in higher cell density with a specific growth rate of 2.49µ. Lipid productivity and lipid % as dry cell weight (DCW) was considerably higher in BL with EPA productivity of 33.4 mg Ld. Fucoxanthin content as % DCW reached 0.95 (BL), 0.75 (RL) and 0.81 (WL) at mid exponential growth phase. The results further prove the plasticity of diatoms and provide a way for future metabolic engineering of T. weissflogii for potential microalgal bio-refinery for combined EPA and FX production.
硅藻可以产生具有生物活性的化合物，如岩藻黄素 (FX) 和二十碳五烯酸 (EPA)，这些化合物在制药和营养保健品行业有很高的需求。在这里，研究了不同光照制度与主要营养物质相结合对海洋硅藻威氏海链藻生长、FX 和 EPA 生产的影响。在两种强度下，威氏海链藻的分批培养在蓝光 (BL)、红光 (RL) 和白光 (WL) 下进行光照。BL 光照制度导致细胞密度更高，比生长速率为 2.49µ。BL 光照制度下的脂生产率和脂%（以干细胞重量 (DCW) 计）明显更高，EPA 生产率为 33.4mg Ld。在指数中期生长阶段，岩藻黄素含量达到 0.95%（BL）、0.75%（RL）和 0.81%（WL）（以 DCW 计）。研究结果进一步证明了硅藻的可塑性，并为未来威氏海链藻的代谢工程提供了一种方法，用于潜在的微藻生物炼制，以同时生产 EPA 和 FX。
