Namour Melanie, Verspecht Tim, El Mobadder Marwan, Teughels Wim, Peremans Andre, Nammour Samir, Rompen Eric
Department of Dental Sciences, Faculty of Medicine, University of Liege, 4000 Liege, Belgium.
Department of Oral Health Sciences, University of Leuven (KU Leuven), Kapucijnenvoer 33, 3000 Leuven, Belgium.
Materials (Basel). 2020 Mar 29;13(7):1573. doi: 10.3390/ma13071573.
(1) Background: The relatively high prevalence of peri-implantitis (PI) and the lack of a standard method for decontamination of the dental implant surface have pushed us to conduct further research in the field. Bacterial biofilms were found to play a primordial role in the etiology of PI. Therefore, the aim is to evaluate the efficacy of a laser-assisted elimination of biofilm protocol in the removal of a multi-species biofilm on titanium surfaces. (2) Methods: In total, 52 titanium discs (grade 4) were used. The study group consisted of 13 titanium disks contaminated with multi-species biofilms and subsequently irradiated with the laser (T + BF + L). The control groups consisted of the following types of titanium disks: 13 contaminated with multi-species biofilms (T + BF), 13 sterile and irradiated (T + L), 13 sterile and untreated (T). Q-Switch Nd:YAG laser Irradiation parameters were the following: energy density equal to 0.597 J/cm per pulse, power equal to 270 milliwatt per pulse, 2.4 mm of spot diameter, and 10 Hz repetition rate for pulse duration of six nanoseconds (ns). The laser irradiation was made during 2 s of total time in non-contact and at 0.5 mm away from the titanium disc surface. After treatment, presence of biofilms on the disks was evaluated by staining with crystal violet (CV), which was measured as optical density at six hundred thirty nm, and statistical analyses were done. (3) Results: the optical density values were 0.004 ± 0.004 for the study group T + BF + L, 0.120 ± 0.039 for group T + BF, 0.006 ± 0.003 for group T + L, and 0.007 ± 0.007 for group T. For the study group, laser treatment resulted in a total elimination of the biofilm, with mean values statistically significantly lower than those of contaminated titanium surfaces and similar to those of sterile titanium surfaces. (4) Conclusions: Our irradiation protocol provided a significant elimination of the multi-species biofilm on titanium surfaces. Laser treated titanium surfaces were biofilm-free, similar to the sterile ones.
(1) 背景：种植体周围炎（PI）的患病率相对较高，且缺乏对牙种植体表面进行去污的标准方法，这促使我们在该领域开展进一步研究。细菌生物膜在种植体周围炎的病因中起着首要作用。因此，本研究旨在评估激光辅助消除生物膜方案对钛表面多物种生物膜的清除效果。(2) 方法：总共使用了52个钛盘（4级）。研究组由13个被多物种生物膜污染并随后接受激光照射的钛盘组成（T + BF + L）。对照组由以下类型的钛盘组成：13个被多物种生物膜污染的（T + BF），13个无菌且接受照射的（T + L），13个无菌且未处理的（T）。调Q Nd:YAG激光照射参数如下：每脉冲能量密度等于0.597 J/cm，每脉冲功率等于270毫瓦，光斑直径2.4 mm，脉冲持续时间为6纳秒（ns）时重复频率为10 Hz。激光照射在非接触状态下进行，总时长2秒，距离钛盘表面0.5 mm。处理后，通过用结晶紫（CV）染色评估盘上生物膜的存在情况，以630 nm处的光密度进行测量，并进行统计分析。(3) 结果：研究组T + BF + L的光密度值为0.004 ± 0.004，T + BF组为0.120 ± 0.039，T + L组为0.006 ± 0.003，T组为0.007 ± 0.007。对于研究组，激光处理导致生物膜完全清除，其平均值在统计学上显著低于被污染的钛表面，且与无菌钛表面相似。(4) 结论：我们的照射方案能显著清除钛表面的多物种生物膜。经激光处理的钛表面无生物膜，与无菌表面类似。
