Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Department of Periodontology, Tufts University School of Dental Medicine, Boston, Massachusetts, USA.
J Periodontol. 2021 Nov;92(11):1613-1621. doi: 10.1002/JPER.20-0765. Epub 2021 Apr 6.
Emergence of peri-implant diseases led to the development of various methods for implant surface decontamination. This study was designed to compare the efficacy of biofilm removal from implant-like titanium surfaces by an erbium-doped yttrium-aluminum-garnet (Er:YAG) laser, titanium brush, and carbon fiber curet.
Eight study subjects were recruited. A custom mouth appliance that held eight sandblasted and acid-etched titanium discs was fabricated for each subject. Subjects were asked to wear this appliance for 72 hours to allow for biofilm development. After retrieval, discs were removed and randomized to one of four treatment groups. The discs were stained with a two-component nucleic acid dye kit, and the residual biofilm was visualized under fluorescence microscopy. Quantification of residual biofilm was performed using an image analysis software and expressed as the percentage surface area.
Fifty-nine titanium discs were randomized to the four treatment groups. The percentage of titanium disc area covered by residual biofilm was 74.0% ± 21.6%, 32.8% ± 24.0%, 11.8% ± 10.3%, and 20.1% ± 19.2% in the control, Er:YAG, titanium brush and carbon fiber curet groups, respectively (mean ± SD). The biofilm-covered area significantly decreased in each of the three treatment groups compared with control (P < 0.008). Comparisons between treatment groups did not reveal statistical significance.
Er:YAG laser treatment is an effective method for reducing the bacterial biofilm on titanium discs. However, on a threadless titanium surface, Er:YAG laser does not exhibit a significantly greater efficacy in biofilm removal than commonly used titanium brushes or carbon fiber curets.
种植体周围疾病的出现促使人们开发了各种种植体表面消毒方法。本研究旨在比较铒激光、钛刷和碳纤维洁治器对类似种植体钛表面生物膜去除的效果。
招募了 8 名研究对象。为每位研究对象制作了一个含有 8 个喷砂酸蚀钛盘的定制口腔器具。要求研究对象佩戴该器具 72 小时以形成生物膜。取出后，将圆盘随机分为四组。用双组分核酸染料试剂盒对圆盘进行染色，荧光显微镜下观察残留生物膜。使用图像分析软件对残留生物膜进行定量，以表面积百分比表示。
随机分配到四组处理的钛盘共 59 个。残留生物膜覆盖的钛盘面积百分比分别为对照组 74.0%±21.6%、铒激光组 32.8%±24.0%、钛刷组 11.8%±10.3%和碳纤维洁治组 20.1%±19.2%（均值±标准差）。与对照组相比，每组的生物膜覆盖面积均显著减少（P<0.008）。各组间比较无统计学意义。
铒激光治疗是减少钛盘细菌生物膜的有效方法。然而，在无螺纹钛表面，与常用的钛刷或碳纤维洁治器相比，铒激光在去除生物膜方面的效果并不显著更好。
