Wawrzyk Anna, Rahnama Mansur, Sofińska-Chmiel Weronika, Wilczyński Sławomir, Gutarowska Beata, Konka Adam, Zeljas Dagmara, Łobacz Michał
Silesian Park of Medical Technology Kardio-Med Silesia in Zabrze, M. Curie Skłodowskiej 10C Str., 41-800 Zabrze, Poland.
Chair and Department of Oral Surgery, Medical University of Lublin, Chodźki 6, 20-093 Lublin, Poland.
Materials (Basel). 2022 Aug 26;15(17):5890. doi: 10.3390/ma15175890.
The paper presents the optimization of diode laser irradiation of corroded dental implants in order to reduce the number of microorganisms associated peri-implantitis. The research included the identification of microorganisms on the surface of removed dental implants in patients with peri-implantitis and the assessment of the biocidal effectiveness of the diode laser against these microorganisms. Laser desorption/mass spectrometry (MALDI-TOF MS) was used to identify microorganisms and metagens were examined by next generation sequencing (NGS). Irradiation was performed with a diode laser with a wavelength of λ = 810, operating mode: 25 W/15.000 Hz/10 μs, average = 3.84 W with the number of repetitions t = 2 × 15 s and t = 3 × 15 s. The structure and surface roughness of the implants were analysed before and after laser irradiation by optical profilometry and optical microscopy with confocal fixation. In total, 16 species of Gram-positive bacteria and 23 species of Gram-negative bacteria were identified on the surface of the implants. A total of 25 species of anaerobic bacteria and 12 species with corrosive potential were detected. After diode laser irradiation, the reduction in bacteria on the implants ranged from 88.85% to 100%, and the reduction in fungi from 87.75% to 96.77%. The reduction in microorganisms in the abutment was greater than in the endosseous fixture. The applied laser doses did not damage, but only cleaned the surface of the titanium implants. After 8 years of embedding, the removed titanium implant showed greater roughness than the 25-year-old implant, which was not exposed to direct influence of the oral cavity environment. The use of a diode laser in an optimised irradiation dose safely reduces the number of microorganisms identified on corroded dental implants in patients with peri-implantitis.
本文介绍了对腐蚀的牙科植入物进行二极管激光照射优化，以减少与种植体周围炎相关的微生物数量。该研究包括对患有种植体周围炎患者取出的牙科植入物表面微生物的鉴定，以及二极管激光对这些微生物的杀菌效果评估。采用激光解吸/质谱（MALDI-TOF MS）鉴定微生物，并通过下一代测序（NGS）检测宏基因组。使用波长λ = 810的二极管激光进行照射，操作模式：25 W/15000 Hz/10 μs，平均功率 = 3.84 W，重复次数t = 2×15 s和t = 3×15 s。通过光学轮廓仪和共聚焦固定光学显微镜对激光照射前后植入物的结构和表面粗糙度进行分析。总共在植入物表面鉴定出16种革兰氏阳性菌和23种革兰氏阴性菌。共检测到25种厌氧菌和12种具有腐蚀潜力的菌。二极管激光照射后，植入物上细菌的减少率在88.85%至100%之间，真菌的减少率在87.75%至96.77%之间。基台上微生物的减少幅度大于骨内种植体。所应用的激光剂量未对钛植入物表面造成损伤，只是进行了清洁。植入8年后取出的钛植入物比未暴露于口腔环境直接影响的25年植入物表面粗糙度更大。以优化的照射剂量使用二极管激光可安全减少患有种植体周围炎患者腐蚀牙科植入物上鉴定出的微生物数量。
