Department of Applied Sciences, University of the West of England, Bristol BS16 1QY, UK.
Department of Bioengineering, University of California, Berkeley, CA 94720, USA.
Molecules. 2020 Mar 30;25(7):1583. doi: 10.3390/molecules25071583.
Aseptic loosening of total joint replacements (TJRs) continues to be the main cause of implant failures. The socioeconomic impact of surgical revisions is hugely significant; in the United Kingdom alone, it is estimated that £135m is spent annually on revision arthroplasties. Enhancing the longevity of titanium implants will help reduce the incidence and overall cost of failed devices. In realising the development of a superior titanium (Ti) technology, we took inspiration from the growing interest in reactive polydopamine thin films for biomaterial surface functionalisations. Adopting a "one-pot" approach, we exposed medical-grade titanium to a mildly alkaline solution of dopamine hydrochloride (DHC) supplemented with (3S)1-fluoro-3-hydroxy-4-(oleoyloxy)butyl-1-phosphonate (FHBP), a phosphatase-resistant analogue of lysophosphatidic acid (LPA). Importantly, LPA and selected LPA analogues like FHBP synergistically cooperate with calcitriol to promote human osteoblast formation and maturation. Herein, we provide evidence that simply immersing Ti in aqueous solutions of DHC-FHBP afforded a surface that was superior to FHBP-Ti at enhancing osteoblast maturation. The facile step we have taken to modify Ti and the biological performance of the final surface finish are appealing properties that may attract the attention of implant manufacturers in the future.
全关节置换术（TJR）的无菌性松动仍然是植入物失败的主要原因。手术翻修的社会经济影响非常巨大；仅在英国，估计每年用于翻修关节成形术的费用就高达 1.35 亿英镑。提高钛植入物的使用寿命将有助于降低设备失效的发生率和总体成本。在实现优越钛（Ti）技术的发展过程中，我们从对用于生物材料表面功能化的反应性聚多巴胺薄膜日益增长的兴趣中获得灵感。我们采用“一锅法”，将医用级钛暴露于多巴胺盐酸盐（DHC）的弱碱性溶液中，该溶液中添加了（3S）1-氟-3-羟基-4-（油酰氧基）丁基-1-膦酸（FHBP），这是一种对脂肪酶有抗性的溶血磷脂酸（LPA）类似物。重要的是，LPA 和 FHBP 等选定的 LPA 类似物与骨化三醇协同合作，促进人成骨细胞的形成和成熟。在此，我们提供的证据表明，只需将 Ti 浸入 DHC-FHBP 的水溶液中，就能获得一种比 FHBP-Ti 更能促进成骨细胞成熟的表面。我们采取的简单修饰 Ti 的步骤和最终表面处理的生物性能是吸引人的特性，这可能会引起未来植入物制造商的关注。
