Yang Lin, Yang Qiang, Li Fan, Yi Wuzhou, Liu Fangfang, Wang Songbo, Jiang Qingyan
Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Wushan Road No.483, Guangzhou 510642, Guangdong, China.
National Engineering Research Center for Breeding Swine Industry and UBT Lipid Suite Functional Fatty Acids Research Center, South China Agricultural University, Wushan Road No.483, Guangzhou 510642, Guangdong, China.
Animals (Basel). 2020 Mar 22;10(3):529. doi: 10.3390/ani10030529.
Our previous studies demonstrated that lauric acid (LA) stimulated mammary gland development during puberty. However, the roles of LA on lactation in mice remain indeterminate. Thus, the aim of this study was to investigate the effects of dietary LA supplementation on lactation functioning and to study the potential mechanisms during lactation. in vivo, there was no effect of 1% LA dietary supplementation during lactation on the feed intake or body weight of breast-feeding mice. However, maternal LA supplementation significantly expanded the number of mammary gland alveoli of mice during lactation and the average body weight of the offspring, suggesting that LA supplementation enhanced the development and lactation function of the mammary glands. in vitro, 100 μM of LA significantly increased the content of triglycerides (TG) in the cell supernatant of induced HC11 cells, however, with no effect on the expression of the genes associated with fatty acid synthesis. LA also activated the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway. LA dietary supplementation significantly expanded the serum levels of lipid metabolites, including sphingomyelin and other metabolites with the sn-2 position of C12 and sn-1 position of C18 in the TG of the lactating mice. Taken together, dietary supplementation of LA during lactation could promote the lactation function of mice, which might be related to increasing the development of the mammary glands and alternation of serum lipid metabolites. These findings provided more theoretical and experimental basis for the application of lauric acid in the development of mammary glands and lactation function of lactating animals.
我们之前的研究表明，月桂酸（LA）在青春期可刺激乳腺发育。然而，LA对小鼠泌乳的作用仍不明确。因此，本研究旨在探讨日粮添加LA对泌乳功能的影响，并研究泌乳期间的潜在机制。在体内，泌乳期间日粮添加1% LA对哺乳小鼠的采食量或体重没有影响。然而，母体补充LA可显著增加泌乳期间小鼠的乳腺腺泡数量以及后代的平均体重，这表明补充LA可增强乳腺的发育和泌乳功能。在体外，100 μM的LA可显著增加诱导的HC11细胞的细胞上清液中甘油三酯（TG）的含量，然而，对与脂肪酸合成相关的基因表达没有影响。LA还激活了磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）信号通路。日粮补充LA可显著提高泌乳小鼠血清脂质代谢产物的水平，包括鞘磷脂以及TG中C12的sn-2位和C18的sn-1位的其他代谢产物。综上所述，泌乳期间日粮补充LA可促进小鼠的泌乳功能，这可能与乳腺发育增加和血清脂质代谢产物改变有关。这些发现为月桂酸在泌乳动物乳腺发育和泌乳功能中的应用提供了更多的理论和实验依据。
