Department of Korean Medicine, Dongshin University, Naju, 58245, Korea.
Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan Jeonnam, 58579, Korea.
Sci Rep. 2020 Mar 31;10(1):5718. doi: 10.1038/s41598-020-62749-7.
Although inflammation is a host defense mechanism, chronic inflammation mediates several diseases, including cancer, allergy, asthma, and autoimmune diseases, and reportedly, it is associated with a 60% mortality rate. There are several reports on the anti-inflammatory effects of Curcuma longa and Allium hookeri. However, although they can be used as culinary materials and have biological effects, they are not effective anti-inflammatory agents. In this study, we evaluated the synergic effect of C. longa and A. hookeri in order to confirm the possibility of a new anti-inflammatory agent. Based on cell viability and cytokine analyses, the appropriate ratio of C. longa and A. hookeri was confirmed using an air pouch animal model. Then, the anti-inflammatory effect of C. longa and A. hookeri co-treatment was evaluated by measuring the immune cell count and cytokines in the exudate and by comparing the morphological changes and cytokines in inflamed skin samples. Additionally, we evaluated the NF-κB/COX-2 pathway and iNOS levels. The active constituents detected in C. longa were demethoxycurcumin and bisdemethoxycurcumin, and that detected in A. hookeri was methylsulfonylmethane. An in vitro assessment determined the appropriate drug ratio as 3:7. In a carrageenan-induced inflammatory model, co-treatment effectively suppressed inflammatory cytokines, including IFN-γ, IL-1β, IL-6, IL-13, and IL-17, and recovered inflammation-related morphological changes in the skin. The anti-inflammatory effect of the co-treatment was mediated through the NF-κB/COX-2 pathway and iNOS inhibition. We concluded that co-treatment with C. longa and A. hookeri synergistically inhibited inflammation via the NF-κB/COX-2/iNOS pathway.
虽然炎症是一种宿主防御机制，但慢性炎症介导了几种疾病，包括癌症、过敏、哮喘和自身免疫性疾病，据报道，它与 60%的死亡率有关。有几份关于姜黄和葱属钩的抗炎作用的报告。然而，尽管它们可以用作烹饪材料并有生物作用，但它们不是有效的抗炎剂。在这项研究中，我们评估了姜黄和葱属钩的协同作用，以确认新型抗炎剂的可能性。基于细胞活力和细胞因子分析，使用气囊动物模型确认了姜黄和葱属钩的适当比例。然后，通过测量渗出液中的免疫细胞计数和细胞因子，并比较炎症皮肤样本中的形态变化和细胞因子，评估姜黄和葱属钩共同治疗的抗炎作用。此外，我们还评估了 NF-κB/COX-2 通路和 iNOS 水平。在姜黄中检测到的活性成分是脱甲氧基姜黄素和双脱甲氧基姜黄素，在葱属钩中检测到的活性成分是甲基磺酰甲烷。体外评估确定了合适的药物比例为 3:7。在角叉菜胶诱导的炎症模型中，共同治疗有效抑制了炎症细胞因子，包括 IFN-γ、IL-1β、IL-6、IL-13 和 IL-17，并恢复了皮肤中与炎症相关的形态变化。共同治疗的抗炎作用是通过 NF-κB/COX-2 通路和 iNOS 抑制介导的。我们得出结论，姜黄和葱属钩的共同治疗通过 NF-κB/COX-2/iNOS 通路协同抑制炎症。
