Wooten-Saadi E, Blecha F, Stevenson J S, Broce A B
Department of Entomology, College of Agriculture, Kansas State University, Manhattan 66506.
Am J Vet Res. 1988 Sep;49(9):1555-9.
Blood samples from sarcoptic mite-infested pigs were evaluated for effects of mite infestation and cold and ambient temperatures on lymphocyte blastogenic responses and for effects of mite infestation on serum cortisol concentrations. In experiment 1, sarcoptic mite-infested and noninfested pigs were housed in cold (5 to 15 C fluctuating) and thermoneural (25 C) environmental chambers for 5 weeks. Differences were not observed (P greater than 0.10) in blastogenic responses to phytohemagglutin or pokeweed mitogen between lymphocytes from infested and noninfested pigs on postinfestation days (PID) 7, 21, 28, and 35 in either environmental chamber. When lymphocytes from noninfested pigs were cultured with sera from infested pigs, alterations of blastogenic responses were not detected. Cortisol values were higher (P less than 0.05) in sera from sarcoptic mite-infested pigs, compared with those from noninfested pigs, at 4 PM on PID 14 and 4 AM and 10 AM on PID 15. Cortisol values were higher (P less than 0.05) in sera obtained at 10 AM on PID 14 and at 10 AM on PID 15 from pigs housed in cold chambers, compared with those from pigs housed in thermoneutral chambers. Interactive effects between sarcoptic mite infestation and cold ambient temperatures were not observed. At 4 AM on PID 15 (experiment 2), cortisol values were higher (P less than 0.05) in sera of infested pigs, compared with those in noninfested pigs. Seemingly, sarcoptic mange in pigs did not alter mitogen-induced lymphocyte blastogenic responses, but did increase serum cortisol concentrations, indicating that sarcoptic mange may be a stressor in pigs.
对感染疥螨的猪的血液样本进行评估，以研究疥螨感染、寒冷和环境温度对淋巴细胞增殖反应的影响，以及疥螨感染对血清皮质醇浓度的影响。在实验1中，将感染疥螨和未感染疥螨的猪置于寒冷（5至15摄氏度波动）和温度适中（25摄氏度）的环境舱中饲养5周。在感染后第7、21、28和35天，无论是在哪个环境舱中，感染猪和未感染猪的淋巴细胞对植物血凝素或商陆有丝分裂原的增殖反应均未观察到差异（P大于0.10）。当用感染猪的血清培养未感染猪的淋巴细胞时，未检测到增殖反应的改变。在感染后第14天下午4点、第15天凌晨4点和上午10点，感染疥螨的猪血清中的皮质醇值高于未感染猪（P小于0.05）。与置于温度适中环境舱中的猪相比，置于寒冷环境舱中的猪在感染后第14天上午10点和第15天上午10点获得的血清中皮质醇值更高（P小于0.05）。未观察到疥螨感染与寒冷环境温度之间的交互作用。在感染后第15天凌晨4点（实验2），感染猪血清中的皮质醇值高于未感染猪（P小于0.05）。似乎猪的疥螨病不会改变有丝分裂原诱导的淋巴细胞增殖反应，但会增加血清皮质醇浓度，这表明猪的疥螨病可能是一种应激源。
