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[金银花配方颗粒快速质量检测系统]

[Rapid quality detection system of Lonicerae Japonicae Flos formula granules].

作者信息

Chen Zi-Yuan, Wang Li, Jiang Chao, Jin Yan, Zhou Jun-Hui, Nan Tie-Gui, Yuan Yuan

机构信息

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2020 Mar;45(5):1070-1075. doi: 10.19540/j.cnki.cjcmm.20200112.103.

Abstract

The application of traditional Chinese medicine(TCM) formula granules in clinical practice is gradually extensive. However, TCM formula granules is still lacking rapid and simple quality control standards. In this study, allele-specific PCR and enzyme-linked immunoassay(ELISA) was used for rapid detection of the quality of Lonicerae Japonicae Flos formula granules. The authenticity of Lonicerae Japonicae Flos formula granules was identified by allele-specific PCR and index component was detected by ELISA. Thus, it lays a foundation for the establishment of rapid quality detection standard for Lonicerae Japonicae Flos formula granules, and also provides reference for other studies on the quality standard of traditional Chinese medicine formula granules.

摘要

中药配方颗粒在临床实践中的应用日益广泛。然而,中药配方颗粒仍缺乏快速简便的质量控制标准。本研究采用等位基因特异性PCR和酶联免疫吸附测定法(ELISA)对金银花配方颗粒的质量进行快速检测。通过等位基因特异性PCR鉴定金银花配方颗粒的真伪,并用ELISA检测指标成分。从而为建立金银花配方颗粒快速质量检测标准奠定了基础,也为其他中药配方颗粒质量标准的研究提供了参考。

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