Zhang Qi, Gao Yan, Wang Yan, Liu Dong-Han, Liu Cai-Feng, Zhong Lin-Ying, Liang Jun, Tian Wan-Wan, Hong Ting-Ting, Bai Jie, DU Shou-Ying
School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
Zhongguo Zhong Yao Za Zhi. 2020 Feb;45(4):878-883. doi: 10.19540/j.cnki.cjcmm.20191001.305.
In this experiment, the decoction process of famous classical formula Xiebai San was determined by optimizing the particle size of "Cuo san" and investigating the decoction process parameters, such as boiling container, water volume and duration. Xiebai San was taken as an example to explore the study method of the "Cuo san" in the famous classical formulas. The specific chromatogram of Xiebai San and the determination method of glycyrrhizin and glycyrrhizic acid in Xiebai San were established. Different particle sizes of "Cuo san" and decoction parameters were optimized based on the similarity of specific chromatogram, the specific chromatogram's peak area, the content of glycyrrhizin, the content of glycyrrhizic acid and extract yield rate.The particle size of Xiebai San powder was determined to be 2.00-4.75 mm(by four-mesh sieves). The decoction process was determined as follows: put the prescription amount into a ceramic pot, add 420 mL of water, and boil and simmer until the volume is 300 mL.The similarity of specific chromatogram was above 0.9, the specific chromatogram's peak area was larger, the content of glycyrrhizin was 0.12%, the content of glycyrrhizic acid was 0.21%,and the extract yield rate was 15.05%. The finally determined particle size of "Cuo san" can better represent the quality of Xiebai San, and is easy to prepare and suitable for industrial production.This experimental research method can comprehensively investigate the quality of Xiebai San as a whole, the content of active ingredients, and the situation of extract yield.It is a more comprehensive and objective evaluation method, and can provide experimental basis and reference for the study of other "Cuo san" famous classical formulas.
本实验通过优化“锉散”的粒度，并考察煎煮容器、加水量、煎煮时间等煎煮工艺参数，确定了著名经典方剂泻白散的煎煮工艺。以泻白散为例，探索著名经典方剂中“锉散”的研究方法。建立了泻白散的特征图谱以及泻白散中甘草苷和甘草酸的测定方法。基于特征图谱的相似度、特征图谱峰面积、甘草苷含量、甘草酸含量及浸出率，对不同粒度的“锉散”及煎煮参数进行优化。确定泻白散散剂的粒度为2.00 - 4.75 mm（四号筛）。确定煎煮工艺如下：按处方量投入陶瓷罐中，加水420 mL，煮沸后小火煎煮至体积为300 mL。特征图谱相似度大于0.9，特征图谱峰面积较大，甘草苷含量为0.12%，甘草酸含量为0.21%，浸出率为15.05%。最终确定的“锉散”粒度能更好地代表泻白散的质量，且易于制备，适合工业化生产。该实验研究方法能全面考察泻白散整体质量、有效成分含量及浸出率情况。是一种较为全面客观的评价方法，可为其他含“锉散”的著名经典方剂研究提供实验依据和参考。
