Xu Jin-Guo, Huang Zi-Yan, Shen Qian-Neng, Li Lin, Wang Qiao-Han, Wang Lu, Lu Tu-Lin, Mao Chun-Qin
School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
Zhongguo Zhong Yao Za Zhi. 2020 Dec;45(23):5599-5606. doi: 10.19540/j.cnki.cjcmm.20200921.301.
Based on fingerprint and network pharmacology,the whole process quality control of Zhuru Decoction was conducted and efficacy-related substances were predicted.The fingerprints of raw materials,decoction pieces and Zhuru Decoction were established,and 25 common peaks were identified,including 9 common chromatographic peaks of 3'-hydroxy puerarin,puerarin,3'-methoxy puerarin,puerarin,aperioside,daidzin,daidzein,liquiritin,glycyrrhizic acid and 6-gingerol, with similarity all greater than 0.95.The main groups of pharmacodynamic substances can be transferred from raw materials,decoction pieces to Zhuru Decoction step by step,with a clear affiliation relationship.Based on the testability and traceability,the active ingredients were screened,and the network relationship of "component-target-pathway" was constructed and analyzed for the nine chemical components screened by network pharmacology.The enriched pathways included energy metabolism,alcoholism,and smooth muscle contraction and relaxation-related pathways.The nine active components of Zhuru Decoction may achieve the effects of clearing heat, alleviating a hangover, harmonizing stomach and stopping vomiting through these signaling pathways.Based on transitive and traceable properties of the above 9 components as well as their close relationship to the efficacy of Zhuru Decoction,these 9 components can be identified as potential efficacy-related substances and provide basis for the overall quality control of Zhuru Decoction.
基于指纹图谱和网络药理学，对竹茹汤进行了全过程质量控制并预测了药效相关物质。建立了原材料、饮片及竹茹汤的指纹图谱，共鉴定出25个共有峰，其中包括3'-羟基葛根素、葛根素、3'-甲氧基葛根素、葛根素、木犀草苷、大豆苷、大豆黄素、甘草苷、甘草酸和6-姜酚9个共有色谱峰，相似度均大于0.95。药效物质主要基团可逐步从原材料、饮片转移至竹茹汤，归属关系明确。基于可测性和可追溯性，筛选出活性成分，构建并分析了网络药理学筛选出的9个化学成分的“成分-靶点-通路”网络关系。富集的通路包括能量代谢、酒精中毒以及平滑肌收缩与舒张相关通路。竹茹汤的9种活性成分可能通过这些信号通路达到清热、解酒、和胃止呕的功效。基于上述9种成分的传递性、可追溯性及其与竹茹汤药效的密切关系，可将这9种成分确定为潜在的药效相关物质，为竹茹汤的整体质量控制提供依据。
