Ibrahim M E, Moussa M A, Pedersen H
Department of Obstetrics and Gynaecology, Faculty of Medicine, Kuwait University, Safat.
Arch Androl. 1988;21(2):129-33. doi: 10.3109/01485018808986723.
Semen samples from husbands with a history of unexplained infertility (n = 33), of women with habitual abortion (n = 36), or normal fertile donors (n = 20) were subjected to conventional semen analysis (SA), Acridine orange test (AOT), and zona-free hamster egg penetration test (HEPT). The three tests operate independently. The most discriminatory test was AOT (p = 0.0001) followed by HEPT (p = 0.019). The frequency of sperm chromatin heterogeneity as detected by AOT red fluorescence was highest in habitual abortion (39.4%), followed by unexplained infertility (16.4%), and, last, donors (9.4%). However the percentage of penetration was highest in habitual abortion (50.7%), followed by donors (43.1%), and least in unexplained infertility (33.9%). Conventional semen parameters (sperm density, motility, abnormality, and vitality) were the least to discriminate between the three groups. The presence of abnormal sperm chromatin may lead to infertility as a result of early pregnancy loss.
选取有不明原因不孕病史的丈夫的精液样本（n = 33）、有习惯性流产史的女性的精液样本（n = 36）或正常可育供者的精液样本（n = 20），进行常规精液分析（SA）、吖啶橙试验（AOT）和无透明带仓鼠卵穿透试验（HEPT）。这三项试验独立进行。最具鉴别力的试验是AOT（p = 0.0001），其次是HEPT（p = 0.019）。通过AOT红色荧光检测到的精子染色质异质性频率在习惯性流产组中最高（39.4%），其次是不明原因不孕组（16.4%），最后是供者组（9.4%）。然而，穿透率在习惯性流产组中最高（50.7%），其次是供者组（43.1%），在不明原因不孕组中最低（33.9%）。常规精液参数（精子密度、活力、形态异常和存活率）在区分这三组时鉴别力最差。异常精子染色质的存在可能由于早期妊娠丢失而导致不孕。
