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吖啶橙荧光法作为男性生育力检测方法。

Acridine orange fluorescence as male fertility test.

作者信息

Ibrahim M E, Pedersen H

机构信息

Department of Obstetrics and Gynaecology, Faculty of Medicine, Kuwait University, Safat.

出版信息

Arch Androl. 1988;20(2):125-9. doi: 10.3109/01485018808987062.

DOI:10.3109/01485018808987062
PMID:3395156
Abstract

Acridine orange fluorescence, semen analysis, and zona-free hamster egg penetration tests were performed on 100 consecutive semen samples. No significant correlation was detected between sperm motility and red fluorescence. However, abnormal sperm morphology correlated significantly with red fluorescence (p less than or equal to 0.001) and fluorescence index (p less than or equal to 0.001). Presence of a high percentage of red fluorescing sperm did not prevent their penetration of zona-free hamster egg, indicating that the presence of abnormal DNA had no effect on the sperm fertility. The significance of acridine orange fluorescence male fertility test requires further elucidation.

摘要

对100份连续的精液样本进行了吖啶橙荧光、精液分析和去透明带仓鼠卵穿透试验。未检测到精子活力与红色荧光之间存在显著相关性。然而,异常精子形态与红色荧光(p≤0.001)和荧光指数(p≤0.001)显著相关。高比例红色荧光精子的存在并不妨碍它们穿透去透明带仓鼠卵,这表明异常DNA的存在对精子生育力没有影响。吖啶橙荧光男性生育力试验的意义需要进一步阐明。

相似文献

1
Acridine orange fluorescence as male fertility test.吖啶橙荧光法作为男性生育力检测方法。
Arch Androl. 1988;20(2):125-9. doi: 10.3109/01485018808987062.
2
Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence.通过吖啶橙荧光评估,与透明带结合的人类精子具有正常的核染色质。
Hum Reprod. 2007 Jun;22(6):1597-602. doi: 10.1093/humrep/dem044. Epub 2007 Mar 16.
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A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence.一种通过吖啶橙(AO)荧光对男性生育能力进行实际评估的测试。
Fertil Steril. 1984 Jul;42(1):87-91. doi: 10.1016/s0015-0282(16)47963-x.
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[The acridine orange test. A new parameter in assessing the fertilizing capacity of spermatozoa].[吖啶橙试验。评估精子受精能力的一个新参数]
Zentralbl Gynakol. 1989;111(20):1361-7.
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Relationship between fertility, semen analysis and human sperm penetration of zona-free hamster eggs.生育能力、精液分析与人类精子穿透去透明带仓鼠卵之间的关系。
Acta Eur Fertil. 1983 Sep-Oct;14(5):331-6.
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Sperm chromatin heterogeneity as an infertility factor.精子染色质异质性作为一种不孕因素。
Arch Androl. 1988;21(2):129-33. doi: 10.3109/01485018808986723.
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Thiol-disulfide status and acridine orange fluorescence of mammalian sperm nuclei.哺乳动物精子细胞核的硫醇-二硫键状态与吖啶橙荧光
J Androl. 1992 Jul-Aug;13(4):342-8.
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The relationship between the human sperm hypoosmotic swelling test, routine semen analysis, and the human sperm zona-free hamster ovum penetration assay.人类精子低渗肿胀试验、常规精液分析与人类精子无透明带仓鼠卵穿透试验之间的关系。
Fertil Steril. 1985 Nov;44(5):668-72. doi: 10.1016/s0015-0282(16)48985-5.
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Zona-free hamster egg-sperm penetration assay: II. Correlative study with routine semen analysis.无透明带仓鼠卵-精子穿透试验:II. 与常规精液分析的相关性研究。
Arch Androl. 1985;14(1):59-63. doi: 10.3109/01485018508987279.
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A comparison between the hamster egg penetration test and the seminal parameters in men of infertile couples.不育夫妇男性的仓鼠卵穿透试验与精液参数的比较。
Int J Fertil. 1988 May-Jun;33(3):207-11.

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Association of various sperm parameters with unexplained repeated early pregnancy loss--which is most important?各种精子参数与不明原因的反复早期妊娠丢失的关联——哪一个最重要?
Int Urol Nephrol. 2008;40(2):391-5. doi: 10.1007/s11255-007-9282-y.
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Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos.
通过吖啶橙染色检测到的精子单链DNA会降低卵胞浆内单精子注射(ICSI)衍生胚胎的受精率和质量。
J Assist Reprod Genet. 2002 Jul;19(7):319-28. doi: 10.1023/a:1016006509036.
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Human protamines and male infertility.人类鱼精蛋白与男性不育症。
J Assist Reprod Genet. 1997 May;14(5):282-90. doi: 10.1007/BF02765830.