Nagy Magdolna, Heemskerk Johan W M, Swieringa Frauke
a Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM) , Maastricht University , Maastricht , The Netherlands.
b Department of Bioanalytics , Leibniz Institute for Analytical Sciences - ISAS- e.V. , Dortmund , Germany.
Platelets. 2017 Jul;28(5):441-448. doi: 10.1080/09537104.2017.1293809. Epub 2017 Mar 30.
This paper provides an overview of the various types of microfluidic devices that are employed to study the complex processes of platelet activation and blood coagulation in whole blood under flow conditions. We elaborate on how these devices are used to detect impaired platelet-dependent fibrin formation in blood from mice or patients with specific bleeding disorders. We provide a practical guide on how to assess formation of a platelet-fibrin thrombus under flow, using equipment that is present in most laboratories. In addition, we describe current insights on how blood flow and shear rate alter the location of platelet populations, von Willebrand factor, coagulation factors, and fibrin in a growing thrombus. Finally, we discuss possibilities and limitations for the clinical use of microfluidic devices to evaluate a hemostatic or prothrombotic tendency in patient blood samples.
本文概述了各种类型的微流控装置，这些装置用于研究在流动条件下全血中血小板活化和血液凝固的复杂过程。我们详细阐述了如何使用这些装置来检测来自患有特定出血性疾病的小鼠或患者血液中血小板依赖性纤维蛋白形成受损的情况。我们提供了一份实用指南，介绍如何使用大多数实验室都有的设备来评估流动状态下血小板 - 纤维蛋白血栓的形成。此外，我们描述了关于血流和剪切速率如何改变正在形成的血栓中血小板群体、血管性血友病因子、凝血因子和纤维蛋白位置的当前见解。最后，我们讨论了微流控装置在临床应用中评估患者血液样本止血或血栓形成倾向的可能性和局限性。
