School of Cancer and Pharmaceutical Sciences, Faculty of Life Science & Medicine, King's College London, UK.
Department of Chemistry and UNAM-National Nanotechnology Research Centre, Bilkent University, Turkey.
Biomater Sci. 2020 May 6;8(9):2590-2599. doi: 10.1039/d0bm00314j.
In this work we describe the formulation and characterisation of red-emitting polymeric nanocapsules (NCs) incorporating superparamagnetic iron oxide nanoparticles (SPIONs) for magnetic tumour targeting. The self-fluorescent oligomers were synthesised and chemically conjugated to PLGA which was confirmed by NMR spectroscopy, FT-IR spectroscopy and mass spectrometry. Hydrophobic SPIONs were synthesised through thermal decomposition and their magnetic and heating properties were assessed by SQUID magnetometry and calorimetric measurements, respectively. Magnetic nanocapsules (m-NCs) were prepared by a single emulsification/solvent evaporation method. Their in vitro cytotoxicity was examined in CT26 colon cancer cells. The formulated fluorescent m-NCs showed good stability and biocompatibility both in vitro and in vivo in CT 26 colon cancer models. Following intravenous injection, accumulation of m-NCs in tumours was observed by optical imaging. A higher iron content in the tumours exposed to a magnetic field, compared to the contralateral tumours without magnetic exposure in the same animal, further confirmed the magnetic tumour targeting in vivo. The overall results show that the engineered red-emitting m-NCs have great potential as multifunctional nanocarriers for multi-model bioimaging and magnetic-targeted drug delivery.
在这项工作中，我们描述了红色发光聚合物纳米胶囊（NCs）的配方和特性，该纳米胶囊包含超顺磁性氧化铁纳米颗粒（SPIONs），用于磁性肿瘤靶向。自荧光低聚物通过化学方法与 PLGA 偶联，这通过 NMR 光谱、FT-IR 光谱和质谱得到证实。通过热分解合成疏水性 SPIONs，并通过 SQUID 磁强计和量热法分别评估其磁性和加热性能。通过单乳化/溶剂蒸发法制备磁性纳米胶囊（m-NCs）。在 CT26 结肠癌细胞中检查其体外细胞毒性。在 CT26 结肠癌细胞模型中，体外和体内实验表明，所制备的荧光 m-NCs 具有良好的稳定性和生物相容性。静脉注射后，通过光学成像观察到 m-NCs 在肿瘤中的积累。与同一动物中未暴露于磁场的对侧肿瘤相比，暴露于磁场的肿瘤中的铁含量更高，这进一步证实了体内的磁性肿瘤靶向。总的来说，结果表明，工程化的红色发光 m-NCs 具有作为多功能纳米载体用于多模式生物成像和磁性靶向药物递送的巨大潜力。
