Terada N, Leiderman L J, Oka T
Biochem Biophys Res Commun. 1983 Mar 29;111(3):1059-65. doi: 10.1016/0006-291x(83)91407-9.
Addition of cortisol at concentrations above 300 nM selectively inhibited the synthesis of alpha-lactalbumin and the accumulation of its mRNA in the mouse mammary gland cultured in the presence of insulin and prolactin, whereas the same treatment augmented casein synthesis and the accumulation of casein mRNA. Prostaglandin E2 or F2 alpha reversed the inhibitory effects of cortisol in a dose-dependent manner, without affecting casein production. The levels of prostaglandin E2 or F2 alpha in tissue explants cultured with insulin and prolactin increased about 2.6-fold over those in uncultured tissue, and the addition of cortisol decreased these levels approximately 2-fold. These results indicate the ability of prostaglandins to counteract the inhibitory effect of cortisol on the alpha-lactalbumin gene expression in the mouse mammary gland.
在存在胰岛素和催乳素的情况下培养的小鼠乳腺中，添加浓度高于300 nM的皮质醇会选择性抑制α-乳白蛋白的合成及其mRNA的积累，而相同处理则会增强酪蛋白的合成和酪蛋白mRNA的积累。前列腺素E2或F2α以剂量依赖性方式逆转皮质醇的抑制作用，而不影响酪蛋白的产生。与未培养组织相比，在胰岛素和催乳素存在下培养的组织外植体中前列腺素E2或F2α的水平增加了约2.6倍，添加皮质醇可使这些水平降低约2倍。这些结果表明前列腺素具有抵消皮质醇对小鼠乳腺中α-乳白蛋白基因表达的抑制作用的能力。
