Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, 143005, India.
Mol Biol Rep. 2020 Apr;47(4):2901-2911. doi: 10.1007/s11033-020-05394-7. Epub 2020 Apr 1.
An endophytic fungus Aspergillus fumigatus isolated from Moringa oleifera has been evaluated for its various bioactivities. The chloroformic fungal extract exhibited a good antimicrobial as well as antibiofilm activity against various pathogenic microorganisms. It also demonstrated a good antimutagenicity against the reactive carcinogenic ester generating mutagen, 2-aminofluorene (2-AF) with IC values of 0.52 mg ml and 0.36 mg ml in case of co-incubation and pre-incubation, respectively. The antiprolifertive activity against different cancer cell lines; such as HCT-15, HeLa A549 and U87-MG showed the IC values of 0.061, 0.065 and 0.072 mg ml, respectively. The antioxidant activity of fungal extract has been assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) (ABTS) methods with IC values of 40.07 µg and 54.28 µg, respectively. Total phenolics and flavonoid contents have been also determined. Ultra-high performance liquid chromatography (UPLC) of fungal extract revealed the presence of various phenolic compounds (caffeic acid, rutin, ellagic acid, quercetin and kaempferol). Further an attempt has been made to purify the bioactive compounds by column chromatography and GC-MS analysis. The above studies demonstrated a good bioactive potential of endophytic fungus Aspergillus fumigatus and shows the pharmacological importance of an endophytic fungus and justify the need to carry out further studies.
从辣木中分离出的内生真菌烟曲霉已被评估其各种生物活性。氯仿真菌提取物对各种致病微生物具有良好的抗菌和抗生物膜活性。它还对反应性致癌酯生成诱变剂 2-氨基芴（2-AF）表现出良好的抗诱变活性，在共孵育和预孵育条件下的 IC 值分别为 0.52mg/ml 和 0.36mg/ml。对不同癌细胞系的抗增殖活性；如 HCT-15、HeLa A549 和 U87-MG 的 IC 值分别为 0.061、0.065 和 0.072mg/ml。真菌提取物的抗氧化活性已通过 2,2-二苯基-1-苦基肼（DPPH）和 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）方法进行评估，IC 值分别为 40.07µg 和 54.28µg。还测定了总酚和类黄酮的含量。真菌提取物的超高效液相色谱（UPLC）显示存在各种酚类化合物（咖啡酸、芦丁、鞣花酸、槲皮素和山奈酚）。进一步尝试通过柱色谱和 GC-MS 分析来纯化生物活性化合物。上述研究表明内生真菌烟曲霉具有良好的生物活性潜力，并显示出内生真菌的药理学重要性，证明需要进一步研究。
