College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China.
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China.
J Microbiol Methods. 2020 May;172:105907. doi: 10.1016/j.mimet.2020.105907. Epub 2020 Mar 30.
Despite menaquinones (MKs)-4 and - 7 being known to have extensive biological activities and applications, less attention has been paid to the other MKs. Thus, to obtain a range of MKs to further explore their pharmacological activities, structure-activity relationships, and applications, a chemical screening method for MK-producing strains was established based on high-performance liquid chromatography-ultraviolet (HPLC-UV) technology. Considering that Bacillus strains have proven to be an important MK-producing bioresource, twenty-nine putative Bacillus isolates previously sought from a fermented soybean sample were used for the validation of the chemical screening method, which ultimately led to the discovery of sixteen MK-producing strains. Among them, Bacillus subtilis DC-1 presented excellent ability to produce MKs. Another, a purchased strain of B. amyloliquefaciens was discovered to be an MK-producing strain. These results indicated this screening method was simple and highly efficient for the discovery of MK-producing strains, especially those producing a range of MK structures.
尽管甲萘醌（MKs）-4 和 -7 已被证实具有广泛的生物学活性和应用，但人们对其他 MKs 的关注较少。因此，为了获得一系列 MKs 以进一步探索它们的药理活性、构效关系和应用，建立了一种基于高效液相色谱-紫外（HPLC-UV）技术的 MK 产生菌株的化学筛选方法。考虑到芽孢杆菌菌株已被证明是一种重要的 MK 产生生物资源，先前从发酵大豆样品中寻找的 29 种可能的芽孢杆菌分离物被用于验证化学筛选方法，最终发现了 16 种 MK 产生菌株。其中，枯草芽孢杆菌 DC-1 表现出优异的产生 MKs 的能力。另一个发现是，一种购买的解淀粉芽孢杆菌菌株是一种 MK 产生菌株。这些结果表明，该筛选方法简单高效，特别适用于发现产生多种 MK 结构的 MK 产生菌株。
