Dulos E, Dufourcq J, Fougnot C, Jozefowicz M
Centre de Recherche Paul Pascal, CNRS, Domaine Universitaire, Talence, France.
Biomaterials. 1988 Sep;9(5):405-12. doi: 10.1016/0142-9612(88)90004-x.
Quenching of fluorescence was used to monitor adsorption of thrombin (T), antithrombin (AT) and their inactive complex (T-AT) onto three anticoagulant biomaterials made of polystyrene beads bearing the functional groups of heparin. An adsorption capacity of 0.12 mumol of T per mg of polymer allowed the formation of a monolayer of protein at the polymer surface. An affinity constant of 3 x 10(7) l.mol-1 between thrombin and polymer was estimated, whatever the polymer used. The affinity of T-AT was similar although weaker. Desorption of proteins from the polymeric interface by means of polycations (polybrene and polylysine) showed that the inactive complex T-AT is more quantitatively and easily released than thrombin.
利用荧光猝灭来监测凝血酶（T）、抗凝血酶（AT）及其无活性复合物（T - AT）在三种由带有肝素官能团的聚苯乙烯珠体制成的抗凝血生物材料上的吸附情况。每毫克聚合物吸附0.12 μmol的T的吸附容量使得在聚合物表面形成了蛋白质单层。无论使用何种聚合物，估计凝血酶与聚合物之间的亲和常数为3×10⁷ l·mol⁻¹。T - AT的亲和力相似，不过较弱。通过聚阳离子（聚凝胺和聚赖氨酸）从聚合物界面解吸蛋白质表明，无活性复合物T - AT比凝血酶更易于定量且更容易释放。
