Boisson C, Jozefonvicz J, Brash J L
Centre Scientifique et Polytechnique, Université, Paris-Nord, France.
Biomaterials. 1988 Jan;9(1):47-52. doi: 10.1016/0142-9612(88)90068-3.
The adsorption of albumin and thrombin to insoluble modified polystyrene resins bearing sulphonate (PSSO3) and L-arginyl methyl ester groups (PAOM) was investigated in both purified and plasma systems. Radioiodinated proteins were used to follow adsorption in a 'minicolumn' experiment. Albumin adsorption was found to follow the Langmuir model and specific surface areas of the various resins were evaluated from plateau albumin adsorption data. The adsorption isotherms of thrombin both in buffer and in antithrombin III/fibrinogen-free plasma were also found to be Langmuir-like, and the quantities adsorbed at the isotherm plateaux are in the monolayer range. Analysis of the isotherms at 4 degrees and 37 degrees C for the purified system shows that adsorption is endothermic. Adsorption capacities in plasma remain high (30-50% of those in the purified system) despite competition from the other plasma proteins. These data confirm the strong affinity and selectivity of these resins for thrombin.
在纯化系统和血浆系统中，研究了白蛋白和凝血酶在带有磺酸根（PSSO3）和L - 精氨酰甲酯基团（PAOM）的不溶性改性聚苯乙烯树脂上的吸附情况。在“微型柱”实验中，使用放射性碘化蛋白质来追踪吸附过程。发现白蛋白吸附符合朗缪尔模型，并根据白蛋白吸附平台数据评估了各种树脂的比表面积。凝血酶在缓冲液和无抗凝血酶III/纤维蛋白原血浆中的吸附等温线也呈类似朗缪尔的形式，等温线平台处的吸附量处于单层范围内。对纯化系统在4℃和37℃下的等温线分析表明，吸附是吸热的。尽管存在其他血浆蛋白的竞争，但血浆中的吸附容量仍然很高（为纯化系统中的30 - 50%）。这些数据证实了这些树脂对凝血酶具有很强的亲和力和选择性。
