Fougnot C, Jozefowicz M, Rosenberg R D
Biomaterials. 1984 Mar;5(2):89-93. doi: 10.1016/0142-9612(84)90007-3.
In the preceding paper, we described results concerning the adsorption of purified thrombin and antithrombin III on two insoluble anticoagulant polystyrene derivatives. We now report similar results obtained in a plasma system. In each case, the purified protein was mixed with fresh platelet poor plasma in order to maintain the same concentrations of all the other plasma proteins. The thrombin molecule was modified by alkyl phosphorylation of the active serine site prior to mixing with plasma. The adsorption of antithrombin was found to be reduced 8 to 9 times when the protein solution was substituted by diluted plasma. In contrast, the thrombin adsorption only depends on the substituents bound on the polymeric chain. These results are supported by those of the study of the competition between purified antithrombin and albumin.
在前一篇论文中，我们描述了关于纯化的凝血酶和抗凝血酶III在两种不溶性抗凝聚苯乙烯衍生物上吸附的结果。我们现在报告在血浆系统中获得的类似结果。在每种情况下，将纯化的蛋白质与新鲜的少血小板血浆混合，以保持所有其他血浆蛋白的浓度相同。在与血浆混合之前，通过活性丝氨酸位点的烷基磷酸化对凝血酶分子进行修饰。当蛋白质溶液被稀释血浆替代时，发现抗凝血酶的吸附减少了8至9倍。相比之下，凝血酶的吸附仅取决于结合在聚合物链上的取代基。纯化的抗凝血酶和白蛋白之间竞争研究的结果支持了这些结果。
