College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu 225009, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu 225009, China.
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Poult Sci. 2020 Apr;99(4):2136-2145. doi: 10.1016/j.psj.2019.12.008. Epub 2020 Feb 28.
This study investigated the prevalence of Salmonella and the molecular typing of all isolates in a goose production chain including hatchery, farm, slaughterhouse, and market. A total of 350 Salmonella isolates was detected from 1,030 samples, and 13 serotypes were recovered. The highest Salmonella contamination frequency was observed at the hatchery, which 51.8% (188/363) of samples were Salmonella positive. S. Potsdam and S. Typhimurium were the 2 most common serotypes. S. Potsdam was most frequently found in the hatchery, while S. Typhimurium was widely distributed in the goose production chain. In general, the antibiotic resistance of Salmonella isolates is low, which isolates from the market is comparatively higher than from other production links indicating a possibility of Salmonella cross-contamination in the market. By the multilocus sequence typing (MLST) analysis, 7 different ST types were identified. ST2039 was the most common ST type, which was mostly found from S. Potsdam isolates in hatchery indicating that S. Potsdam might have been long existed in hatchery. The pulsed-field gel electrophoresis (PFGE) analysis of S. Potsdam indicated that S. Potsdam could be transmitted along the production chain. The PFGE analysis of S. Typhimurium showed that PFGE pattern 29 (PF29) was distributed in hatchery, and also in farm and from humans indicating the risk of S. Typhimurium transmitting to humans by the food supply chain. Our study provided the evidence of Salmonella cross-contamination in the slaughterhouse and the retail market of goose production chain, and specific serotypes existed for a long time at a particular production link. The spread of Salmonella along the production chain, might cause harm to humans through cross-contamination. Further studies would be needed to control the Salmonella contamination in hatchery and prevent the transmission of the pathogen during the goose production.
本研究调查了包括孵化场、农场、屠宰场和市场在内的鹅生产链中沙门氏菌的流行情况和所有分离株的分子分型。从 1030 个样本中检测到 350 株沙门氏菌分离株，共回收了 13 种血清型。孵化场的沙门氏菌污染频率最高，363 份样本中有 51.8%（188/363）为沙门氏菌阳性。最常见的血清型为 S.波茨坦和 S. Typhimurium。S.波茨坦在孵化场最常见，而 S. Typhimurium则广泛分布于鹅生产链中。一般来说，沙门氏菌分离株的抗生素耐药性较低，但市场来源的分离株相对较高，表明沙门氏菌在市场上可能存在交叉污染。通过多位点序列分型（MLST）分析，共鉴定出 7 种不同的 ST 型。ST2039 是最常见的 ST 型，主要发现于孵化场的 S.波茨坦分离株中，表明 S.波茨坦可能长期存在于孵化场中。S.波茨坦的脉冲场凝胶电泳（PFGE）分析表明，S.波茨坦可沿生产链传播。S. Typhimurium 的 PFGE 分析表明，PFGE 型 29（PF29）分布于孵化场，也分布于农场，并从人群中分离出来，表明 S. Typhimurium 通过食物链传播给人类的风险。本研究提供了沙门氏菌在屠宰场和鹅生产链零售市场交叉污染的证据，并且特定的血清型在特定的生产环节长期存在。沙门氏菌沿生产链的传播，可能通过交叉污染对人类造成危害。需要进一步研究来控制孵化场的沙门氏菌污染，并防止病原体在鹅生产过程中的传播。
