Department of Acupuncture and Massage, Hebei University of Chinese Medicine, Shijiazhuang 050020, China.
Department of integrated Traditional Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050020, China.
J Tradit Chin Med. 2020 Apr;40(2):212-223.
To investigate the relationship between symptom patterns of cold coagulation and blood stasis (CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction (WJD) for the treatment of CCBS.
CCBS was induced in rats with an ice-water bath treatment. The ovarian function, microvascular and circulatory status of reproductive organs, and function of local microvascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs) were evaluated.
Ovarian dysfunction was observed in the rats with CCBS. It was characterized by the presence of an estrous cycle disorder and a decrease in reproductive hormone levels. Microvascular circulation disorders were associated with an imbalance in vasoconstriction, relaxation substances, nitric oxide, abnormal blood flow in whole blood, and decreased blood flow in the auricle and uterus. VECs were damaged, and VSMCs contracted and proliferated in ovarian and uterine tissues.
Our findings suggest that the dysfunctional reproductive organs observed in gynecological CCBS may be closely related to the microcirculation disturbance of local tissues, microvascular contraction, and vascular remodeling. Modified WJD can be used to treat CCBS by improving microcirculation in reproductive organs.
探讨寒凝血瘀（CCBS）证症状与微循环障碍的关系，并确定加味温经汤（WJD）治疗 CCBS 的疗效。
采用冰水浴处理诱导大鼠 CCBS，评估卵巢功能、生殖器官微血管和循环状态，以及局部微血管内皮细胞（VEC）和血管平滑肌细胞（VSMC）的功能。
CCBS 大鼠出现卵巢功能障碍，表现为动情周期紊乱和生殖激素水平下降。微血管循环障碍与血管收缩、松弛物质、一氧化氮失衡、全血血流异常和耳、子宫血流量减少有关。卵巢和子宫组织中 VEC 受损，VSMC 收缩和增殖。
本研究结果提示，妇科 CCBS 中观察到的生殖器官功能障碍可能与局部组织微循环障碍、微血管收缩和血管重塑密切相关。加味温经汤可通过改善生殖器官微循环治疗 CCBS。
