Scheetz A J, Markham J A, Fifková E
Department of Psychology, University of Colorado, Boulder 80309-0345.
Brain Res. 1988 Sep 20;460(2):246-52. doi: 10.1016/0006-8993(88)90369-1.
The effect of chronic ethanol administration on the density of basket cells in the dentate gyrus of mice selectively bred for their sensitivity to acute ethanol exposure (long-sleep, LS and short-sleep, SS) was assessed in two experiments. In addition, the effect of chronic ethanol on the density of dentate granule cells and astrocytes was examined. In the first experiment, mice received 3 weeks of a liquid ethanol diet with 35% of their calories derived from ethanol (EDC). In this experiment, LS mice did not demonstrate a change in the density of granule cells or in the density of basket cells. There was, however, a significant increase in the density of astrocytes as a result of this treatment for the LS mice. The SS mice were unaffected on all measures. In the second experiment, portions of which have been reported previously, mice received a diet with 23% EDC for 3 months. As a result of this exposure, LS mice showed a significant decrease in the density of basket cells, but there was no change in the density of granule cells or astrocytes. There was no difference between controls and experimental mice from the SS group on any of these parameters. These results suggest that at least in the dentate gyrus, chronic ethanol treatment selectively reduces the density of basket cells but only in mice that are more sensitive to the hypnotic effects of acute ethanol exposure. Furthermore, this effect seems to be preceded by an apparent increase in the density of astrocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
在两项实验中，评估了长期给予乙醇对因对急性乙醇暴露敏感而被选择性培育的小鼠（长睡眠型，LS和短睡眠型，SS）齿状回中篮状细胞密度的影响。此外，还检测了长期给予乙醇对齿状颗粒细胞和星形胶质细胞密度的影响。在第一个实验中，小鼠接受为期3周的液体乙醇饮食，其35%的热量来自乙醇（乙醇饮食组，EDC）。在该实验中，LS小鼠的颗粒细胞密度或篮状细胞密度未显示出变化。然而，这种处理使LS小鼠的星形胶质细胞密度显著增加。SS小鼠在所有指标上均未受影响。在第二个实验中（部分内容先前已报道），小鼠接受含23% EDC的饮食3个月。由于这种暴露，LS小鼠的篮状细胞密度显著降低，但颗粒细胞或星形胶质细胞的密度没有变化。SS组的对照小鼠和实验小鼠在任何这些参数上均无差异。这些结果表明，至少在齿状回中，长期乙醇处理选择性地降低了篮状细胞的密度，但仅在对急性乙醇暴露的催眠作用更敏感的小鼠中如此。此外，在这种效应出现之前，星形胶质细胞的密度似乎有明显增加。（摘要截断于250字）
