Scheetz A J, Markham J A, Fifková E
Brain Res. 1987 Apr 21;409(2):329-34. doi: 10.1016/0006-8993(87)90718-9.
The effect of chronic ethanol administration on the fine structure of the hippocampal CA1 stratum oriens was examined in two lines of mice selectively bred for their differential sensitivity to acute ethanol exposure (long-sleep, LS and short-sleep, SS mice). Two experiments were performed. In the first experiment, mice received a liquid diet for 3 weeks with the final amount of ethanol being 35% ethanol-derived calories. In the second experiment, mice received 23.5% ethanol-derived calories for 3 months. Quantitative electron microscopy of the dendritic spines and synaptic appositions in the stratum oriens of CA1 revealed an interaction between diet and line of mice, but only in the 3-month exposure condition. This difference was due to a significant decrease in the density of spines and synaptic appositions in the LS mice receiving ethanol. Additionally, baseline differences between lines indicate that the lines are differing in the density of spine synapses in the absence of ethanol treatment. The possible interaction between acute sensitivity to ethanol and differences in fine structure are examined.
在两组因对急性乙醇暴露敏感性不同而经选择性培育的小鼠（长睡眠小鼠，LS和短睡眠小鼠，SS）中，研究了长期给予乙醇对海马CA1区海马下托精细结构的影响。进行了两项实验。在第一个实验中，小鼠接受液体饮食3周，最终乙醇含量为35%乙醇衍生热量。在第二个实验中，小鼠接受23.5%乙醇衍生热量，持续3个月。对CA1区海马下托中树突棘和突触附着进行的定量电子显微镜检查显示，饮食与小鼠品系之间存在相互作用，但仅在3个月暴露条件下存在。这种差异是由于接受乙醇的LS小鼠中棘突和突触附着的密度显著降低。此外，品系之间的基线差异表明，在未进行乙醇处理的情况下，品系在棘突突触密度上存在差异。研究了对乙醇的急性敏感性与精细结构差异之间可能存在的相互作用。
