Molecular Parasitology and Entomology Unit, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Special Mission for Eradication of Tsetse flies, Regional tsetse Division of Adamawa, MINEPIA, Ngaoundere, Cameroon.
Vet Med Sci. 2020 Aug;6(3):410-420. doi: 10.1002/vms3.264. Epub 2020 Apr 3.
Brucellosis is one of the world's most widespread bacterial zoonoses caused by Brucella. It leads to considerable economic losses as a result of low productivity of infected animals and the long debilitating illness in humans. Despite its impact on human and animal health, little attention has been paid on Brucella infections in domestic animals. It is in this light that the prevalence of Brucella antibodies was determined in domestic animals with the overarching goal of improving our knowledge on brucellosis in southern Cameroon. During cross-sectional studies conducted from December 2016 to August 2018 in five sites of southern Cameroon, blood samples were collected in cattle, sheep, goat, pig and dog. Plasma was obtained from each blood sample and Brucella antibodies were detected using the Rose Bengal test and the enzyme-linked immunosorbent assay (ELISA). From 1873 animals that were sampled, the overall prevalence of Brucella antibodies using Indirect enzyme-linked immunosorbent assay (i-ELISA) was 6.35% (118/1873): 9.12% (78/855) in cattle; 8.04% (30/373) in sheep; 6.06% (2/33) in dog, 1.87% (3/160) in pig and 1.1% (5/452) in goat. Between animal species (p-value < .0001, x = 33.63) as well as sampling sites (p-value = .0001, x = 18.97), significant differences were observed in the prevalence of Brucella antibodies. Yoko and Noun localities have shown the highest prevalence of 8.6% (30/348) and 7.2% (78/1070), respectively. This prevalence was significantly higher (p = .03, x = 1.25) in female than male cattle. Between adult (16.923%) and young cattle (7.8%), significant difference (p = .04, x = 6.42) was observed in the prevalence of Brucella antibodies. This study shows that the prevalence of Brucella antibodies varies between animal species and localities. It also shows several domestic animals of southern Cameroon that have been in contact with Brucella. It enabled to identify villages where investigations on the transmission dynamic must be focused for the final goal of developing control measures for this neglected zoonotic disease.
布鲁氏菌病是由布鲁氏菌引起的世界上分布最广泛的细菌性人畜共患病之一。由于感染动物的生产力低下和人类长期患病，它导致了相当大的经济损失。尽管它对人类和动物健康有影响，但对家畜布鲁氏菌感染的关注很少。正是出于这一考虑，我们确定了在喀麦隆南部的家畜中布鲁氏菌抗体的流行情况，目的是提高我们对喀麦隆南部布鲁氏菌病的认识。在 2016 年 12 月至 2018 年 8 月期间在喀麦隆南部五个地点进行的横断面研究中，采集了牛、绵羊、山羊、猪和狗的血液样本。从每份血样中提取血浆，并用虎红平板凝集试验和酶联免疫吸附试验（ELISA）检测布鲁氏菌抗体。在 1873 只被采样的动物中，间接酶联免疫吸附试验（i-ELISA）检测到的布鲁氏菌抗体总流行率为 6.35%（118/1873）：牛 9.12%（78/855）；绵羊 8.04%（30/373）；狗 6.06%（2/33）；猪 1.87%（3/160）；山羊 1.1%（5/452）。在不同动物物种（p 值<0.0001，x²=33.63）和采样地点（p 值=0.0001，x²=18.97）之间，布鲁氏菌抗体的流行率存在显著差异。Yoko 和 Noun 两个地方的流行率最高，分别为 8.6%（30/348）和 7.2%（78/1070）。雌性牛的流行率明显更高（p=0.03，x²=1.25）。在成年牛（16.923%）和小牛（7.8%）之间，布鲁氏菌抗体的流行率存在显著差异（p=0.04，x²=6.42）。本研究表明，布鲁氏菌抗体的流行率在不同动物物种和不同地区之间存在差异。它还表明，喀麦隆南部的一些家畜与布鲁氏菌有过接触。这有助于确定必须集中调查传播动态的村庄，以便最终为这种被忽视的人畜共患病制定控制措施。
