Høeg Tracy B, Chmiel Kenneth, Warrick Alexandra E, Taylor Sandra L, Weiss Robert H
Department of Physical Medicine & Rehabilitation, University of California, Sacramento, CA 95817, USA.
Mountain View Rehabilitation Medical Associates, Grass Valley, CA 95945, USA.
Sports (Basel). 2020 Apr 1;8(4):44. doi: 10.3390/sports8040044.
The purpose of this study was to identify plasma metabolites associated with superior endurance running performance. In 2016, participants at the Western States Endurance Run (WSER), a 100-mile (161-km) foot race, underwent non-targeted metabolomic testing of their post-race plasma. Metabolites associated with faster finish times were identified. Based on these results, runners at the 2017 WSER underwent targeted metabolomics testing, including lipidomics and choline levels. The 2017 participants' plasma metabolites were correlated with finish times and compared with non-athletic controls. In 2016, 427 known molecules were detected using non-targeted metabolomics. Four compounds, all phosphatidylcholines (PCs) were associated with finish time (False Discovery Rate (FDR) < 0.05). All were higher in faster finishers. In 2017, using targeted PC analysis, multiple PCs, measured pre- and post-race, were higher in faster finishers (FDR < 0.05). The majority of PCs was noted to be higher in runners (both pre- and post-race) than in controls (FDR < 0.05). Runners had higher choline levels pre-race compared to controls ( < 0.0001), but choline level did not differ significantly from controls post-race ( = 0.129). Choline levels decreased between the start and the finish of the race ( < 0.0001). Faster finishers had lower choline levels than slower finishers at the race finish ( = 0.028).
本研究的目的是确定与卓越耐力跑表现相关的血浆代谢物。2016年，参加西部各州耐力跑（WSER，一项100英里（161公里）的徒步比赛）的选手在赛后接受了血浆的非靶向代谢组学检测。确定了与更快完赛时间相关的代谢物。基于这些结果，2017年参加WSER的选手接受了靶向代谢组学检测，包括脂质组学和胆碱水平检测。将2017年参与者的血浆代谢物与完赛时间进行关联，并与非运动员对照组进行比较。2016年，使用非靶向代谢组学检测到427种已知分子。四种化合物，均为磷脂酰胆碱（PC），与完赛时间相关（错误发现率（FDR）<0.05）。在完赛较快的选手中，这些化合物的含量均更高。2017年，通过靶向PC分析，在完赛较快的选手中，赛前和赛后测量的多种PC含量更高（FDR<0.05）。大多数PC在选手（赛前和赛后）中的含量高于对照组（FDR<0.05）。与对照组相比，选手在赛前的胆碱水平更高（<0.0001），但赛后胆碱水平与对照组无显著差异（=0.129）。在比赛开始到结束之间，胆碱水平下降（<0.0001）。在比赛结束时，完赛较快的选手胆碱水平低于完赛较慢的选手（=0.028）。
