Pawełkowicz Magdalena Ewa, Skarzyńska Agnieszka, Sroka Małgorzata, Szwacka Maria, Pniewski Tomasz, Pląder Wojciech
Department of Plant Genetics, Breeding & Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warsaw, Poland.
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska Street 34, 60-479 Poznan, Poland.
Genes (Basel). 2020 Mar 20;11(3):334. doi: 10.3390/genes11030334.
Transgenic plants are commonly used in breeding programs because of the various features that can be introduced. However, unintended effects caused by genetic transformation are still a topic of concern. This makes research on the nutritional safety of transgenic crop plants extremely interesting. Cucumber ( L.) is a crop that is grown worldwide. The aim of this study was to identify and characterize differentially expressed genes and regulatory miRNAs in transgenic cucumber fruits that contain the gene, which encodes the sweet-tasting protein thaumatin II, by NGS sequencing. We compared the fruit transcriptomes and miRNomes of three transgenic cucumber lines with wild-type cucumber. In total, we found 47 differentially expressed genes between control and all three transgenic lines. We performed the bioinformatic functional analysis and gene ontology classification. We also identified 12 differentially regulated miRNAs, from which three can influence the two targets (assigned as DEGs) in one of the studied transgenic lines (line 224). We found that the transformation of cucumber with and expression of the transgene had minimal impact on gene expression and epigenetic regulation by miRNA, in the cucumber fruits.
由于转基因植物能够引入多种特性，因此常用于育种计划。然而，基因转化引起的非预期效应仍是一个备受关注的话题。这使得转基因作物的营养安全性研究极具吸引力。黄瓜（Cucumis sativus L.）是一种在全球广泛种植的作物。本研究的目的是通过二代测序（NGS）鉴定和表征含有编码甜味蛋白奇异果甜蛋白II基因的转基因黄瓜果实中差异表达的基因和调控性微小RNA（miRNA）。我们比较了三个转基因黄瓜品系与野生型黄瓜的果实转录组和微小RNA组。总共，我们在对照和所有三个转基因品系之间发现了47个差异表达基因。我们进行了生物信息学功能分析和基因本体分类。我们还鉴定出12个差异调控的微小RNA，其中三个可以影响所研究的一个转基因品系（224品系）中的两个靶标（指定为差异表达基因）。我们发现，用该基因转化黄瓜并使其转基因表达对黄瓜果实中的基因表达和微小RNA介导的表观遗传调控影响最小。
