Biomedical Nanotechnology Group, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Methods Mol Biol. 2020;2135:213-221. doi: 10.1007/978-1-0716-0463-2_12.
The increasing applications of quantum dots (QDs) as optic tools in life science have stimulated researchers to evaluate the effects of these nanoprobes in cell viability using a variety of methods, especially colorimetric ones. One of the most applied tests is the MTT assay. In comparison to MTT, for example, the resazurin-based method has the main advantage of not evaluating the cells directly, thus eliminating false-positive results that may arise from the overlap of the absorbances of the QD with the colorimetric compound. Therefore, herein, we describe the resazurin assay as an alternative, simple, quick, sensitivity, reproducible, and nontoxic test to evaluate the in vitro cell viability after QD exposure. Moreover, this test presents an additional advantage; the cells remain viable for complementary experimental procedures, such as cell migration or adhesion.
量子点（QD）作为生命科学中的光学工具的应用越来越广泛，这促使研究人员使用各种方法，特别是比色法，来评估这些纳米探针对细胞活力的影响。其中最常用的测试之一是 MTT 测定法。与 MTT 相比，例如，基于 Resazurin 的方法的主要优点是不直接评估细胞，从而消除了可能由于 QD 的吸光度与比色化合物重叠而产生的假阳性结果。因此，在这里，我们将 Resazurin 测定法描述为一种替代方法，简单，快速，灵敏，可重复且无毒的测试，可评估 QD 暴露后体外细胞活力。此外，该测试具有另一个优点；细胞仍然保持活力，可用于补充实验程序，例如细胞迁移或黏附。
