Fernández-Minotre Lafitte, Montero-Aguilar Mauricio, Vázquez-Vázquez Febe Carolina, Serrano-Bello Janeth, Vega-Baudrit José, Pereira-Reyes Reinaldo, Pozos-Guillén Amaury, Chavarría-Bolaños Daniel
Dentistry Graduate Program, Universidad de Costa Rica, San José 11501-2060, Costa Rica.
Laboratorio de Materiales Dentales, DEPeI-Facultad de Odontología, UNAM, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico.
Int J Mol Sci. 2025 Jun 23;26(13):6018. doi: 10.3390/ijms26136018.
This study aimed to develop polylactic acid (PLA)-based membranes incorporating tramadol (TMD) using air jet spinning (AJS), ensuring stable physicochemical properties and biocompatibility. Two groups were fabricated: 10% PLA membranes (control) and 10% PLA membranes loaded with TMD in an 80:1 ratio (experimental). Characterization included scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV-VIS), and biocompatibility assays with human osteoblasts using resazurin, crystal violet staining, and 5-chloromethylfluorescein diacetate for fluorescence microscopy. SEM revealed a homogeneous, randomly distributed fiber pattern, with diameters under 5 µm and no structural voids. DSC and TGA indicated that TMD was uniformly incorporated, increased the thermal capacity, and slightly lowered the onset and inflection degradation temperatures. FT-IR confirmed the chemical compatibility of TMD with PLA, showing no structural alterations. UV-VIS detected sustained TMD release over 72 h. Biocompatibility tests showed no cytotoxic effects; cell viability and proliferation in TMD-loaded membranes were comparable to controls. Statistical analysis used ANOVA and Wilcoxon tests. 10% PLA membranes loaded with TMD at an 80:1 ratio exhibited stable physicochemical characteristics and favorable biocompatibility, supporting their potential use in drug delivery systems.
本研究旨在利用空气喷射纺丝（AJS）技术制备含有曲马多（TMD）的聚乳酸（PLA）基膜，确保其具有稳定的物理化学性质和生物相容性。制备了两组膜：10%PLA膜（对照组）和按80:1比例负载TMD的10%PLA膜（实验组）。表征方法包括扫描电子显微镜（SEM）、差示扫描量热法（DSC）、热重分析（TGA）、傅里叶变换红外光谱（FT-IR）、紫外可见光谱（UV-VIS），以及使用刃天青、结晶紫染色和5-氯甲基荧光素二乙酸酯进行荧光显微镜观察，对人成骨细胞进行生物相容性检测。SEM显示纤维图案均匀、随机分布，直径小于5µm且无结构空隙。DSC和TGA表明TMD被均匀掺入，增加了热容量，并略微降低了起始降解温度和拐点降解温度。FT-IR证实了TMD与PLA的化学相容性，未显示结构改变。UV-VIS检测到TMD在72小时内持续释放。生物相容性测试显示无细胞毒性作用；负载TMD的膜中的细胞活力和增殖与对照组相当。统计分析采用方差分析和Wilcoxon检验。按80:1比例负载TMD的10%PLA膜表现出稳定的物理化学特性和良好的生物相容性，支持其在药物递送系统中的潜在应用。
