Roushangar Zineh Babak, Shabgard Mohammad Reza, Roshangar Leila, Jahani Kamal
Mechanical Engineering Department, University of Tabriz, Iran.
Mechanical Engineering Department, University of Tabriz, Iran.
J Biomech. 2020 May 7;104:109764. doi: 10.1016/j.jbiomech.2020.109764. Epub 2020 Mar 22.
The growing usage of printed bio scaffolds in the field of regenerative medicine has made this field very important in biomedical engineering. In this regard, three-dimensional printing (3D) technique needs bio-materials with higher mechanical and biological performance. The biomaterials with high mechanical performance beside its bio compatibility are limited. A novel bio-material made of Alginate, Hyaluronic acid, Halloysite Nanotube and Polyvinylidene Fluoride was used and characterized for printing cartilage bio scaffolds through numerical studies. CaCl2 was used for crosslinking of biomaterial. Scanning Electron Microscopy, mechanical tests (tensile and compressive test), MTT assay were conducted for evaluating this novel biomaterial. Different structures of bio material were simulated for numerical studies. The numerical study was performed in ANSYS 18 using three parameter Mooney-Rivlin model. According to experimental and numerical results, Halloysite Nanotube increases the tensile and compressive strength of biomaterial up to 47%. Results show that biomaterial have good mechanical performance due to mechanical forces required for cartilage bio scaffolds besides its high biological performance. Polyvinylidene fluoride reduces the mechanical performance while increasing the cell viability. MTT assay results performed on day 0, day 2 and day 6 show increase in cell number to be about twice for biomaterial containing 40 mg/ml alginate, 40 mg/ml halloysite nanotube, 10 mg/ml hyaluronic acid and 1 w/v Polyvinylidene fluoride. Numerical simulation shows high mechanical performance of bio material in different scaffolds structure. The best structure of bio scaffolds was achieved with 0.4 mm nozzle diameter and 0.4 space between rows.
印刷生物支架在再生医学领域的使用日益增加，这使得该领域在生物医学工程中变得非常重要。在这方面，三维打印（3D）技术需要具有更高机械性能和生物学性能的生物材料。除了生物相容性之外，具有高机械性能的生物材料是有限的。一种由藻酸盐、透明质酸、埃洛石纳米管和聚偏二氟乙烯制成的新型生物材料被用于通过数值研究来打印软骨生物支架并对其进行表征。氯化钙用于生物材料的交联。进行了扫描电子显微镜、机械测试（拉伸和压缩测试）、MTT 分析以评估这种新型生物材料。对生物材料的不同结构进行了数值模拟研究。数值研究是在 ANSYS 18 中使用三参数 Mooney-Rivlin 模型进行的。根据实验和数值结果，埃洛石纳米管可将生物材料的拉伸和压缩强度提高高达 47%。结果表明，除了具有高生物学性能外，由于软骨生物支架所需的机械力，生物材料具有良好的机械性能。聚偏二氟乙烯在提高细胞活力的同时降低了机械性能。在第 0 天、第 2 天和第 6 天进行的 MTT 分析结果表明，对于含有 40mg/ml 藻酸盐、40mg/ml 埃洛石纳米管、10mg/ml 透明质酸和 1w/v 聚偏二氟乙烯的生物材料，细胞数量增加了约两倍。数值模拟表明生物材料在不同支架结构中具有高机械性能。使用 0.4mm 喷嘴直径和行间距 0.4 时可实现生物支架的最佳结构。
