非神经表面外胚层玫瑰花结的形成和F-肌动蛋白动力学驱动哺乳动物神经管闭合。
Non-neural surface ectodermal rosette formation and F-actin dynamics drive mammalian neural tube closure.
作者信息
Zhou Chengji J, Ji Yu, Reynolds Kurt, McMahon Moira, Garland Michael A, Zhang Shuwen, Sun Bo, Gu Ran, Islam Mohammad, Liu Yue, Zhao Tianyu, Hsu Grace, Iwasa Janet
机构信息
Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children & University of California at Davis, School of Medicine, Sacramento, CA, 95817, USA; Department of Biochemistry and Molecular Medicine, School of Medicine, University of California at Davis, Sacramento, CA, 95817, USA.
Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children & University of California at Davis, School of Medicine, Sacramento, CA, 95817, USA; Department of Biochemistry and Molecular Medicine, School of Medicine, University of California at Davis, Sacramento, CA, 95817, USA.
出版信息
Biochem Biophys Res Commun. 2020 Jun 4;526(3):647-653. doi: 10.1016/j.bbrc.2020.03.138. Epub 2020 Apr 2.
The mechanisms underlying mammalian neural tube closure remain poorly understood. We report a unique cellular process involving multicellular rosette formation, convergent cellular protrusions, and F-actin cable network of the non-neural surface ectodermal cells encircling the closure site of the posterior neuropore, which are demonstrated by scanning electron microscopy and genetic fate mapping analyses during mouse spinal neurulation. These unique cellular structures are severely disrupted in the surface ectodermal transcription factor Grhl3 mutants that exhibit fully penetrant spina bifida. We propose a novel model of mammalian neural tube closure driven by surface ectodermal dynamics, which is computationally visualized.
哺乳动物神经管闭合的潜在机制仍知之甚少。我们报告了一个独特的细胞过程，该过程涉及多细胞玫瑰花结形成、趋同细胞突起以及围绕后神经孔闭合部位的非神经表面外胚层细胞的F-肌动蛋白电缆网络，这在小鼠脊髓神经形成过程中通过扫描电子显微镜和遗传命运图谱分析得到了证实。这些独特的细胞结构在表现出完全显性脊柱裂的表面外胚层转录因子Grhl3突变体中严重破坏。我们提出了一个由表面外胚层动力学驱动的哺乳动物神经管闭合的新模型，并通过计算进行了可视化。
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