Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, 1375 Panda Road, Chenghua District, Sichuan Province, 610081, China.
Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, 1375 Panda Road, Chenghua District, Sichuan Province, 610081, China.
Zoology (Jena). 2020 Jun;140:125775. doi: 10.1016/j.zool.2020.125775. Epub 2020 Mar 23.
Mesenchymal stem cells (MSC) have strong therapeutic potential due to their capacity for self-renewal and multilineage differentiation. MSCs can also be useful in preserving the current genetic diversity of endangered wildlife. To date, MSCs from various species have been studied, but only a few species of endangered wild animals have been reported. Adult bone marrow (BM) is a rich source of mesenchymal stem cells. The aim of this study was to isolate and characterize MSCs derived from the BM of red pandas. Red panda BM-MSCs isolated from five individuals were fibroblast-like cells, similar to other species. Cultured BM-MSCs with normal karyotype were negative for the hematopoietic line marker CD34 and the endothelial cell marker CD31 but were positive for MSC markers, including CD44, CD105 and CD90. RT-PCR and western blot analysis showed self-renewal and pluripotency genes, including Oct4, Sox2 and Klf4, were also expressed in red panda BM-MSCs. Finally, red panda BM-MSCs had the potential for differentiation into osteogenic, adipogenic and neuron-like cells by using a combination of previously reported protocols for other species. We have therefore demonstrated that cells harvested from red panda bone marrow are capable of extensive in vitro multiplication and multilineage differentiation, which is an essential step toward their use in the preservation of red pandas biological diversity and future studies on MSC applications in endangered species.
间充质干细胞（MSC）由于其自我更新和多能分化的能力，具有很强的治疗潜力。MSC 还可用于保护濒危野生动物的现有遗传多样性。迄今为止，已经研究了来自各种物种的 MSC，但只有少数几种濒危野生动物有报道。成人骨髓（BM）是间充质干细胞的丰富来源。本研究的目的是从红熊猫的 BM 中分离和鉴定 MSC。从五个人体中分离出的红熊猫 BM-MSC 呈成纤维细胞样，与其他物种相似。具有正常核型的培养 BM-MSC 对造血谱系标记物 CD34 和内皮细胞标记物 CD31 呈阴性，但对 MSC 标记物呈阳性，包括 CD44、CD105 和 CD90。RT-PCR 和 Western blot 分析表明，红熊猫 BM-MSC 中还表达自我更新和多能性基因，包括 Oct4、Sox2 和 Klf4。最后，通过使用先前报道的其他物种的组合方案，红熊猫 BM-MSC 具有分化为成骨细胞、脂肪细胞和类神经元细胞的潜力。因此，我们证明了从红熊猫骨髓中采集的细胞能够进行广泛的体外增殖和多能分化，这是将其用于保护红熊猫生物多样性和未来研究 MSC 在濒危物种中的应用的重要步骤。
