Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), CSIC, 28040 Madrid, Spain.
Int J Mol Sci. 2022 Aug 18;23(16):9319. doi: 10.3390/ijms23169319.
The European mink () is one of Europe's most endangered species, and it is on the brink of extinction in the Iberian Peninsula. The species' precarious situation requires the application of new ex situ conservation methodologies that complement the existing ex situ and in situ conservation measures. Here, we report for the first time the establishment of a biobank for European mink mesenchymal stem cells (emMSC) and oocytes from specimens found dead in the Iberian Peninsula, either free or in captivity. New emMSC lines were isolated from different tissues: bone marrow (emBM-MSC), oral mucosa (emOM-MSc), dermal skin (emDS-MSC), oviduct (emO-MSc), endometrium (emE-MSC), testicular (emT-MSC), and adipose tissue from two different adipose depots: subcutaneous (emSCA-MSC) and ovarian (emOA-MSC). All eight emMSC lines showed plastic adhesion, a detectable expression of characteristic markers of MSCs, and, when cultured under osteogenic and adipogenic conditions, differentiation capacity to these lineages. Additionally, we were able to keep 227 Cumulus-oocyte complexes (COCs) in the biobank, 97 of which are grade I or II. The European mink MSC and oocyte biobank will allow for the conservation of the species' genetic variability, the application of assisted reproduction techniques, and the development of in vitro models for studying the molecular mechanisms of infectious diseases that threaten the species' precarious situation.
欧洲貂 () 是欧洲最濒危的物种之一，在伊比利亚半岛濒临灭绝。该物种岌岌可危，需要应用新的离体保护方法，以补充现有的离体和就地保护措施。在这里，我们首次报告了从在伊比利亚半岛发现的死亡标本（无论是自由的还是圈养的）中建立欧洲貂间充质干细胞 (emMSC) 和卵母细胞生物库。从不同组织中分离出新的 emMSC 系：骨髓 (emBM-MSC)、口腔黏膜 (emOM-MSc)、皮肤真皮 (emDS-MSC)、输卵管 (emO-MSc)、子宫内膜 (emE-MSC)、睾丸 (emT-MSC) 和来自两个不同脂肪库的脂肪组织：皮下 (emSCA-MSC) 和卵巢 (emOA-MSC)。所有 8 种 emMSC 系均表现出塑性粘附，可检测到 MSC 特征标志物的表达，并且在成骨和成脂条件下培养时，具有向这些谱系分化的能力。此外，我们能够在生物库中保存 227 个卵丘-卵母细胞复合体 (COC)，其中 97 个为 I 级或 II 级。欧洲貂 MSC 和卵母细胞生物库将允许保存该物种的遗传变异性，应用辅助生殖技术，并开发体外模型来研究威胁该物种岌岌可危状况的传染病的分子机制。
