Molecular Genetics and Epigenomics Laboratory, College of Agriculture, Science and Technology (CAST), Delaware State University, Dover, DE 19901, USA.
Panhandle Research Extension and Education Center, University of Nebraska, 4502 Avenue I, Scottsbluff, NE 69361, USA.
Biomolecules. 2024 Jan 15;14(1):109. doi: 10.3390/biom14010109.
This study identified proteomic changes in the seeds of two tolerant (SB-DT3 and SB-DT2) and two sensitive (Merlot and Stampede) common bean genotypes in response to terminal drought stress. Differentially expressed proteins (DEPs) were abundant in the susceptible genotype compared to the tolerant line. DEPs associated with starch biosynthesis, protein-chromophore linkage, and photosynthesis were identified in both genotypes, while a few DEPs and enriched biological pathways exhibited genotype-specific differences. The tolerant genotypes uniquely showed DEPs related to sugar metabolism and plant signaling, while the sensitive genotypes displayed more DEPs involved in plant-pathogen interaction, proteasome function, and carbohydrate metabolism. DEPs linked with chaperone and signal transduction were significantly altered between both genotypes. In summary, our proteomic analysis revealed both conserved and genotype-specific DEPs that could be used as targets in selective breeding and developing drought-tolerant common bean genotypes.
本研究鉴定了两个耐干旱（SB-DT3 和 SB-DT2）和两个敏感（Merlot 和 Stampede）普通菜豆基因型种子对终末干旱胁迫的蛋白质组变化。与耐干旱品种相比，敏感品种中富含大量差异表达蛋白（DEPs）。在两个基因型中均鉴定到与淀粉生物合成、蛋白-发色团连接和光合作用相关的 DEPs，而少数 DEPs 和富集的生物途径表现出基因型特异性差异。耐干旱品种特异地显示与糖代谢和植物信号转导相关的 DEPs，而敏感品种则显示更多与植物-病原体相互作用、蛋白酶体功能和碳水化合物代谢相关的 DEPs。与伴侣蛋白和信号转导相关的 DEPs 在两个基因型之间发生显著变化。总之，我们的蛋白质组学分析揭示了保守和基因型特异性的 DEPs，可作为选择性育种和开发耐旱普通菜豆基因型的目标。
