Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, People's Republic of China.
J Mater Chem B. 2020 May 14;8(18):4106-4121. doi: 10.1039/c9tb02754h. Epub 2020 Apr 7.
In the tissue engineering of cartilage, scaffolds with appropriate elasticity and controlled-release properties are essential. Herein, we synthesized a poly(ether-ester-urethane)urea scaffold with a pendant amino group (PEEUUN) through a de-protection process from PEEUU-Boc polymers and grafted kartogenin (KGN) onto the PEEUUN scaffolds (PEEUUN-KGN). Characterization, performance tests, scaffold biocompatibility analysis, and chondrogenesis evaluation both in vitro and in vivo were conducted. The results revealed that the PEEUUN-KGN scaffolds were degradable and three-dimensional (3D) with interconnected pores, and possessed good elasticity, as well as excellent cytocompatibility. Meanwhile, KGN on the PEEUUN-KGN scaffolds underwent stable sustained release for a long time and promoted human umbilical cord mesenchymal stem cells (HUCMSCs) to differentiate into chondrocytes in vitro, thus enhancing cartilage regeneration in vivo. In conclusion, the present PEEUUN-KGN scaffolds would have application potential for cartilage tissue engineering.
在软骨组织工程中，具有适当弹性和控制释放性能的支架是必不可少的。在此，我们通过脱保护过程从 PEEUU-Boc 聚合物合成了带有侧基氨基的聚（醚酯-氨酯）脲支架（PEEUUN），并将卡托金（KGN）接枝到 PEEUUN 支架上（PEEUUN-KGN）。我们对其进行了表征、性能测试、支架生物相容性分析以及体内外的软骨生成评估。结果表明，PEEUUN-KGN 支架具有可降解性、三维（3D）连通多孔结构、良好的弹性以及优异的细胞相容性。同时，PEEUUN-KGN 支架上的 KGN 能够长时间稳定持续释放，促进人脐带间充质干细胞（HUCMSCs）向软骨细胞分化，从而增强体内软骨再生。综上所述，本研究中的 PEEUUN-KGN 支架在软骨组织工程中有应用潜力。
