Zhao Yanhong, Zhao Xige, Zhang Rui, Huang Ying, Li Yunjie, Shan Minhui, Zhong Xintong, Xing Yi, Wang Min, Zhang Yang, Zhao Yanmei
Stomatological Hospital of Tianjin Medical University, Tianjin, China.
Tianjin Medical University, Tianjin, China.
Front Bioeng Biotechnol. 2020 Dec 9;8:600103. doi: 10.3389/fbioe.2020.600103. eCollection 2020.
Repair of articular cartilage defects is a challenging aspect of clinical treatment. Kartogenin (KGN), a small molecular compound, can induce the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into chondrocytes. Here, we constructed a scaffold based on chondrocyte extracellular matrix (CECM) and poly(lactic-co-glycolic acid) (PLGA) microspheres (MP), which can slowly release KGN, thus enhancing its efficiency. Cell adhesion, live/dead staining, and CCK-8 results indicated that the PLGA(KGN)/CECM scaffold exhibited good biocompatibility. Histological staining and quantitative analysis demonstrated the ability of the PLGA(KGN)/CECM composite scaffold to promote the differentiation of BMSCs. Macroscopic observations, histological tests, and specific marker analysis showed that the regenerated tissues possessed characteristics similar to those of normal hyaline cartilage in a rabbit model. Use of the PLGA(KGN)/CECM scaffold may mimic the regenerative microenvironment, thereby promoting chondrogenic differentiation of BMSCs and . Therefore, this innovative composite scaffold may represent a promising approach for acellular cartilage tissue engineering.
关节软骨缺损的修复是临床治疗中一个具有挑战性的方面。小分子化合物卡托金（KGN）可诱导骨髓间充质干细胞（BMSC）分化为软骨细胞。在此，我们构建了一种基于软骨细胞外基质（CECM）和聚乳酸-乙醇酸共聚物（PLGA）微球（MP）的支架，其可缓慢释放KGN，从而提高其效率。细胞黏附、活/死染色及CCK-8结果表明PLGA（KGN）/CECM支架具有良好的生物相容性。组织学染色和定量分析证明了PLGA（KGN）/CECM复合支架促进BMSC分化的能力。大体观察、组织学检测及特异性标志物分析表明，在兔模型中再生组织具有与正常透明软骨相似的特征。使用PLGA（KGN）/CECM支架可模拟再生微环境，从而促进BMSC的软骨形成分化。因此，这种创新的复合支架可能是一种有前途的无细胞软骨组织工程方法。
