Liu Qiang, Liu Yanghong, He Hongping, Wang Fan, Yao Danyu, He Fangfei, Liu Haifeng, Fan Yubo
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Xue Yuan Road No. 37, Haidian District, Beijing 100083, People's Republic of China.
J Mater Chem B. 2018 Feb 7;6(5):769-780. doi: 10.1039/c7tb02774e. Epub 2018 Jan 19.
Fullerene has been investigated for use in intratracheal instillation and inhalation. However, its use may be compromised due to the formation of reactive oxygen species (ROS) from a long-term stored water-soluble fullerene system, which will result in pulmonary injury. In this study, we investigated the ability of different concentrations of silk fibroin (SF) to scavenge hydroxyl radicals (OH˙) produced by a water-soluble fullerene system. In addition, we elaborated the mechanism of OH˙ formation and scavenging, the degradation of water-soluble fullerene (WSF), and the effects of OH˙ and WSF on the viability of endothelial cells (ECs). WSF was found to rapidly degrade when incubated with SF at 4 °C, which suggested that OH˙ and the deposition of WSF over 5 half-lives might be reduced by mixing WSF with SF. Moreover, it was observed that OH˙ and WSF could generate adverse effects on EC viability, and OH˙ produced by the WSF system on day 55 could be scavenged by SF. Overall, this study indicated that SF as an antioxidant was capable of scavenging OH˙ and accelerating the degradation of WSF, which provides further insight into the application of WSF in intratracheal instillation and inhalation.
富勒烯已被研究用于气管内滴注和吸入。然而，由于长期储存的水溶性富勒烯系统会形成活性氧（ROS），其使用可能会受到影响，这将导致肺损伤。在本研究中，我们研究了不同浓度的丝素蛋白（SF）清除水溶性富勒烯系统产生的羟基自由基（OH˙）的能力。此外，我们阐述了OH˙形成和清除的机制、水溶性富勒烯（WSF）的降解以及OH˙和WSF对内皮细胞（ECs）活力的影响。发现WSF在4℃与SF孵育时会迅速降解，这表明通过将WSF与SF混合，可能会减少OH˙以及超过5个半衰期的WSF沉积。此外，观察到OH˙和WSF会对EC活力产生不利影响，并且第55天WSF系统产生的OH˙可被SF清除。总体而言，本研究表明SF作为一种抗氧化剂能够清除OH˙并加速WSF的降解，这为WSF在气管内滴注和吸入中的应用提供了进一步的见解。
