Shepard N, Mitchell N
Anat Rec. 1977 Apr;187(4):463-76. doi: 10.1002/ar.1091870404.
Rabbit articular cartilage was fixed with glutaraldehyde containing Ruthenium Red or Safranin O proteoglycan localization is easily obtained. Ruthenium Red stained proteoglycan is easily visualized by electron microscopy. Previous digestion with papain prevented staining by either technique confirming that the material was indeed proteoglycan. Using these methods of proteoglycan identification the sites of attachment to collagen are shown as well as the deposition of proteoglycan about cells of differing vitality and the relationship of proteoglycan to cell membranes.
兔关节软骨用含钌红或番红O的戊二醛固定，可轻松获得蛋白聚糖定位。钌红染色的蛋白聚糖通过电子显微镜很容易观察到。先前用木瓜蛋白酶消化可防止两种技术染色，证实该物质确实是蛋白聚糖。使用这些蛋白聚糖鉴定方法，可显示其与胶原蛋白的附着位点、不同活力细胞周围蛋白聚糖的沉积情况以及蛋白聚糖与细胞膜的关系。
