Wang Rong-Gui, Zhao Meng-Yao, Deng Di, Ye Xin, Zhang Fan, Chen Hui, Kong Ji-Lie
Department of Chemistry, Fudan University, Shanghai 200433, China.
J Mater Chem B. 2018 Jul 28;6(28):4592-4601. doi: 10.1039/c8tb00931g. Epub 2018 Jul 6.
Photodynamic therapy (PDT) has been widely applied in cancer treatment due to minimal invasion, negligible side effects and specific tumor ablation. However, the treatment efficiency has been hindered by hypoxia in solid tumors, hydrophobic photosensitizers and their real time tracking. In this paper, we constructed an intelligent and biocompatible bovine serum albumin (BSA)-Ce6-Si QDs-MnO (BCSM NPs) nanocomplex as a pH/HO responsive photosensitizer nanocarrier to modulate tumor hypoxia for fluorescence imaging-guided efficient PDT. This versatile nanosystem not only enhanced the loading capacity of the photosensitizer and the formation of cytotoxic singlet oxygen (O) owing to the intelligent production of oxygen catalyzed by MnO from the endogenous HO, but also performed as a dual functional fluorescence and Magnetic Resonance imaging (MRI) probe. In vivo experiments in nude mice further confirmed that BCSM NPs significantly inhibited the growth of HeLa bearing-tumors compared to free Ce6. The results highlight the great potentail of multifunctional BCSM NPs for in vivo imaging as well as enhancing the photodynamic therapy efficiency.
光动力疗法（PDT）因其微创、副作用可忽略不计以及能特异性消融肿瘤而被广泛应用于癌症治疗。然而，实体瘤中的缺氧、疏水性光敏剂及其实时追踪问题阻碍了治疗效率。在本文中，我们构建了一种智能且生物相容的牛血清白蛋白（BSA）-Ce6-硅量子点-MnO（BCSM纳米粒子）纳米复合物，作为一种pH/过氧化氢响应性光敏剂纳米载体，用于调节肿瘤缺氧情况，以实现荧光成像引导下的高效光动力疗法。这种多功能纳米系统不仅由于MnO从内源性过氧化氢催化产生氧气的智能过程而提高了光敏剂的负载能力并促进了细胞毒性单线态氧（O）的形成，还作为一种双功能荧光和磁共振成像（MRI）探针发挥作用。裸鼠体内实验进一步证实，与游离Ce6相比，BCSM纳米粒子显著抑制了荷HeLa肿瘤的生长。这些结果突出了多功能BCSM纳米粒子在体内成像以及提高光动力治疗效率方面的巨大潜力。
