Du Xiaoyu, Yu Bin, Pei Peng, Ding Huifeng, Yu Baoqing, Zhu Yufang
School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai, 200093, China.
J Mater Chem B. 2018 Jan 21;6(3):499-509. doi: 10.1039/c7tb02667f. Epub 2018 Jan 5.
The development of biomaterials with high osteogenic ability for fast osteointegration with a host bone is of great interest. In this study, pearl/CaSO composite scaffolds were fabricated using three-dimensional (3D) printing, followed by a hydration process. The pearl/CaSO scaffolds showed uniform interconnected macropores (∼400 μm), high porosity (∼60%), and enhanced compressive strength. With CaSO scaffolds as a control, the biological properties of the pearl/CaSO scaffolds were evaluated in vitro and in vivo. The results showed that the pearl/CaSO scaffolds possessed a good apatite-forming ability and stimulated the proliferation and differentiation of rat bone mesenchymal stem cells (rBMSCs), as well as giving a better expression of related osteogenic genes. Importantly, micro-computed tomography and histology of the critical-sized rabbit femoral condyle defects implanted with the scaffolds illustrated the osteogenic capacity of the pearl/CaSO scaffolds. New bone was observed within 8 weeks. The bone-implant contact index was significantly higher for the pearl/CaSO scaffolds implant than for the CaSO scaffolds implant, indicating that the pearl/CaSO scaffolds would be promising implants for bone regeneration.
开发具有高成骨能力、能与宿主骨快速骨整合的生物材料备受关注。在本研究中，采用三维（3D）打印制备珍珠/CaSO复合支架，随后进行水化处理。珍珠/CaSO支架呈现出均匀相连的大孔（约400μm）、高孔隙率（约60%）以及增强的抗压强度。以CaSO支架作为对照，对珍珠/CaSO支架的生物学特性进行了体外和体内评估。结果表明，珍珠/CaSO支架具有良好的磷灰石形成能力，能刺激大鼠骨髓间充质干细胞（rBMSCs）的增殖和分化，还能使相关成骨基因有更好的表达。重要的是，对植入该支架的临界尺寸兔股骨髁缺损进行的微计算机断层扫描和组织学检查表明了珍珠/CaSO支架的成骨能力。在8周内观察到了新骨。珍珠/CaSO支架植入物的骨-植入物接触指数显著高于CaSO支架植入物，这表明珍珠/CaSO支架有望成为骨再生植入物。
