Wang Tiandi, Zheng Jianchao, Hu Tianzhou, Zhang Hongbo, Fu Kun, Yin Ruixue, Zhang Wenjun
Complex and Intelligent Research Center, East China University of Science and Technology (ECUST), Shanghai, P.R. China.
Hainan Hospital, Haikou, P.R. China.
3D Print Addit Manuf. 2021 Feb 1;8(1):1-13. doi: 10.1089/3dp.2020.0140. Epub 2021 Feb 16.
Three-dimensional (3D) printing technology has been applied to fabricate bone tissue engineering scaffolds for a wide range of materials with precisely control over scaffold structures. Coral is a potential bone repair and bone replacement material. Due to the natural source limitation of coral, we developed a fabrication protocol for 3D printing of calcium carbonate (CaCO) nanoparticles for coral replacement in the application of bone tissue engineering. Up to 80% of CaCO nanoparticles can be printed with high resolution using poly-l-lactide as a blender. The scaffolds were subjected to a controlled hydrothermal process for incomplete conversion of carbonate to phosphate to produce CaCO scaffold covered by hydroxyapatite (HA) to modify the biocompatibility and degradation of CaCO/HA scaffolds. X-ray diffraction and Fourier transform infrared spectroscopy showed that HA was converted and attached to the surface of the scaffold, and the surface morphology and microstructure were studied using a scanning electron microscope. To confirm the bone regeneration performance of the scaffold, cell proliferation and osteogenic differentiation of MC3T3 cells on the scaffold were evaluated. In addition, experiments showed that CaCO/HA scaffolds can promote bone growth and repairing process and has high potential in bone tissue engineering. ClinicalTrials.gov ID: SH9H-2020-A603.
三维（3D）打印技术已被应用于制造骨组织工程支架，可用于多种材料，能精确控制支架结构。珊瑚是一种潜在的骨修复和骨替代材料。由于珊瑚的天然来源有限，我们开发了一种用于3D打印碳酸钙（CaCO）纳米颗粒的制造方案，用于在骨组织工程应用中替代珊瑚。使用聚-L-丙交酯作为混合剂，高达80%的CaCO纳米颗粒可以高分辨率打印。对支架进行受控水热过程，使碳酸盐不完全转化为磷酸盐，以制备覆盖有羟基磷灰石（HA）的CaCO支架，从而改善CaCO/HA支架的生物相容性和降解性能。X射线衍射和傅里叶变换红外光谱表明，HA已转化并附着在支架表面，并使用扫描电子显微镜研究了表面形态和微观结构。为了确认支架的骨再生性能，评估了MC3T3细胞在支架上的细胞增殖和成骨分化。此外，实验表明CaCO/HA支架可以促进骨生长和修复过程，在骨组织工程中具有很高的潜力。ClinicalTrials.gov标识符：SH9H-2020-A603。
