Ma Fei, Li Chen-Chen, Zhang Chun-Yang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
J Mater Chem B. 2018 Oct 21;6(39):6173-6190. doi: 10.1039/c8tb01869c. Epub 2018 Sep 3.
Development of efficient biosensors for sensitive and selective detection of specific biomolecules is crucial to both fundamental biomedical research and clinical diagnosis. Due to their unique and superior optical and electronic properties, such as high brightness, good photostability, broad absorption spectrum, narrow and size-tunable emission spectrum, large Stokes shift, versatile surface modification, and distinctive photoelectrochemical activity, semiconductor quantum dots (QDs) have been regarded as promising and attractive building blocks for the development of efficient biosensors with high sensitivity, good selectively, rapidity and simplicity. In this review, we summarize the progress of QD-based biosensors in the last 5 years (2013-2018) including QD-based fluorescent, bioluminescent, chemiluminescent, photoelectrochemical biosensors, and focus on their basic principles and their applications for the detection of DNAs, microRNAs, proteins, enzymes, and living cells. Moreover, we give new insight into the future direction and challenges in the development of QD-based biosensors.
开发用于灵敏且选择性地检测特定生物分子的高效生物传感器，对于基础生物医学研究和临床诊断均至关重要。半导体量子点（QDs）因其独特且优异的光学和电子特性，如高亮度、良好的光稳定性、宽吸收光谱、窄且可调节尺寸的发射光谱、大斯托克斯位移、多样的表面修饰以及独特的光电化学活性，而被视为用于开发具有高灵敏度、良好选择性、快速性和简易性的高效生物传感器的有前景且具吸引力的构建模块。在本综述中，我们总结了过去5年（2013 - 2018年）基于量子点的生物传感器的进展，包括基于量子点的荧光、生物发光、化学发光、光电化学生物传感器，并着重介绍了它们的基本原理及其在检测DNA、微小RNA、蛋白质、酶和活细胞方面的应用。此外，我们对基于量子点的生物传感器开发的未来方向和挑战给出了新的见解。
