基于量子点的用于 microRNA 分析的生物传感器的研究进展：综述。

Progress in quantum dot-based biosensors for microRNA assay: A review.

机构信息

School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China.

出版信息

Anal Chim Acta. 2023 Oct 16;1278:341615. doi: 10.1016/j.aca.2023.341615. Epub 2023 Jul 13.

DOI:10.1016/j.aca.2023.341615

Abstract

MicroRNAs (miRNAs) are responsible for post-transcriptional gene regulation, and may function as valuable biomarkers for diseases diagnosis. Accurate and sensitive analysis of miRNAs is in great demand. Quantum dots (QDs) are semiconductor nanomaterials with superior optoelectronic features, such as high quantum yield and brightness, broad absorption and narrow emission, long fluorescence lifetime, and good photostability. Herein, we give a comprehensive review about QD-based biosensors for miRNA assay. Different QD-based biosensors for miRNA assay are classified by the signal types including fluorescent, electrochemical, electrochemiluminescent, and photoelectrochemical outputs. We highlight the features, principles, and performances of the emerging miRNA biosensors, and emphasize the challenges and perspectives in this field.

摘要

微小 RNA（miRNA）负责转录后基因调控，并且可以作为疾病诊断有价值的生物标志物。因此，对 miRNA 进行准确而灵敏的分析是非常有需求的。量子点（QD）是具有优越光电特性的半导体纳米材料，例如高量子产率和亮度、宽吸收和窄发射、长荧光寿命和良好的光稳定性。在此，我们全面回顾了基于 QD 的 miRNA 分析生物传感器。根据信号类型（包括荧光、电化学、电致化学发光和光电化学输出）对基于 QD 的 miRNA 分析生物传感器进行分类。我们突出了新兴 miRNA 生物传感器的特点、原理和性能，并强调了该领域的挑战和前景。