Gomez I Jennifer, Arnaiz Blanca, Cacioppo Michele, Arcudi Francesca, Prato Maurizio
Carbon Bionanotechnology Laboratory, CIC biomaGUNE, Paseo de Miramón 182, 20014 Donostia-San Sebastián, Spain.
J Mater Chem B. 2018 Sep 21;6(35):5540-5548. doi: 10.1039/c8tb01796d. Epub 2018 Aug 29.
Carbon nanodots (CNDs) hold great potential in imaging and drug delivery applications. In this study, nitrogen-doped CNDs (NCNDs) were coupled to the anticancer agent paclitaxel (PTX) through a labile ester bond. NCNDs showed excellent cell viability and endowed the NCND-PTX conjugate with good water solubility. The hybrid integrates the optical properties of the nanodots with the anticancer function of the drug into a single unit. Cytotoxicity was evaluated in breast, cervix, lung, and prostate cancer cell lines by the MTT assay while the cellular uptake was monitored using confocal microscopy. NCND-PTX induced apoptosis in cancer cells exhibiting slightly better anticancer activity compared to the drug alone. Moreover, the course of the NCND-PTX interaction with cancer cells was monitored using an xCELLigence system. The NCND-based conjugate represents a promising platform for bioimaging and drug delivery.
碳纳米点（CNDs）在成像和药物递送应用中具有巨大潜力。在本研究中，氮掺杂碳纳米点（NCNDs）通过不稳定的酯键与抗癌药物紫杉醇（PTX）偶联。NCNDs表现出优异的细胞活力，并赋予NCND-PTX偶联物良好的水溶性。该杂化物将纳米点的光学性质与药物的抗癌功能整合到一个单一单元中。通过MTT法在乳腺癌、宫颈癌、肺癌和前列腺癌细胞系中评估细胞毒性，同时使用共聚焦显微镜监测细胞摄取情况。与单独使用药物相比，NCND-PTX诱导癌细胞凋亡，表现出略好的抗癌活性。此外，使用xCELLigence系统监测NCND-PTX与癌细胞相互作用的过程。基于NCND的偶联物是生物成像和药物递送的一个有前景的平台。
