University of Science and Technology of China, Hefei 230026, China.
J Mater Chem B. 2019 Apr 28;7(16):2608-2612. doi: 10.1039/c9tb00274j. Epub 2019 Mar 20.
DNA tetrahedron-modified silver nanoparticles (AgNPs) were achieved via amino-silver chemistry for the first time and were applied as a colorimetric biosensor for detecting HIV-related DNA. Target DNA initiated strand displacement polymerization and nicking endonuclease-aided cycles were involved to link DNA tetrahedron-modified AgNPs, reporting colorimetric responses. This developed method showed excellent specificity and sensitivity. A wide linear range from 1 to 15 000 nM was achieved with a limit of detection of 0.84 nM. Moreover, it was successfully applied to determine DNA in blood serum samples.
首次通过氨基银化学法制备了 DNA 四面体修饰的银纳米粒子（AgNPs），并将其用作检测 HIV 相关 DNA 的比色生物传感器。目标 DNA 引发链置换聚合和切口内切酶辅助循环，将 DNA 四面体修饰的 AgNPs 连接起来，报告比色响应。该方法具有优异的特异性和灵敏度。线性范围从 1 到 15000 nM，检测限为 0.84 nM。此外，它还成功地应用于测定血清样本中的 DNA。
