Liu Jiangjun, Yu Meng, Zeng Guobo, Cao Jie, Wang Yuanhe, Ding Tao, Yang Xu, Sun Kang, Parvizi Javad, Tian Shaoqi
Medical College, Qingdao University, Qingdao, 266071, China.
J Mater Chem B. 2018 Dec 21;6(47):7854-7861. doi: 10.1039/c8tb02493f. Epub 2018 Nov 14.
A periprosthetic joint infection (PJI) with methicillin-resistant Staphylococcus aureus (MRSA) is a catastrophic deep joint infection for patients who have had joint replacement surgery. To efficiently eradicate the MRSA, curcumin-upconversion nanoparticles (curcumin-UCNPs) were synthesized by binding the curcumin onto UCNPs, which could produce singlet oxygen under near infrared (NIR) irradiation. Dual antibacterial behavior induced by the curcumin-UCNP itself and induced by photodynamic therapy were demonstrated. Nearly 100% MRSA was eradicated using curcumin-UCNPs under the NIR irradiation because of the dual antibacterial behavior in vitro. Furthermore, a MRSA-induced PJI model was constructed using Sprague Dawley rats. The NIR could penetrate the rats' knee joint tissue well and activated the curcumin-UCNPs to perform photodynamic therapy, which exhibited a good antibacterial effect in the deep joint tissue (1 cm) and about 80% of MRSA was eradicated in vivo. Blood tests and histopathological examinations revealed that the inflammation was also significantly relieved because of the eradication of MRSA in the curcumin-UCNPs-NIR group. Compared with traditional treatments, this research may provide a new therapy for PJI.
耐甲氧西林金黄色葡萄球菌（MRSA）引起的人工关节周围感染（PJI）对于接受关节置换手术的患者来说是一种灾难性的深部关节感染。为了有效根除MRSA，通过将姜黄素结合到上转换纳米颗粒（UCNPs）上合成了姜黄素-上转换纳米颗粒（curcumin-UCNPs），其在近红外（NIR）照射下可产生单线态氧。证明了curcumin-UCNP本身诱导的双重抗菌行为以及光动力疗法诱导的双重抗菌行为。由于体外的双重抗菌行为，在NIR照射下使用curcumin-UCNPs可根除近100%的MRSA。此外，使用Sprague Dawley大鼠构建了MRSA诱导的PJI模型。NIR可以很好地穿透大鼠膝关节组织并激活curcumin-UCNPs进行光动力疗法，在深部关节组织（1厘米）中表现出良好的抗菌效果，并且在体内根除了约80%的MRSA。血液检测和组织病理学检查显示，由于curcumin-UCNPs-NIR组中MRSA的根除，炎症也得到了显著缓解。与传统治疗方法相比，本研究可能为PJI提供一种新的治疗方法。
