Wang Qing, Jiao Xiaojie, Liu Chang, He Song, Zhao Liancheng, Zeng Xianshun
School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
J Mater Chem B. 2018 Jun 28;6(24):4096-4103. doi: 10.1039/c8tb00646f. Epub 2018 Jun 5.
Nitric oxide, an important cellular messenger molecule, plays a wide range of physiological roles in neurotransmission, vascular relaxation, platelet activation, and immune response. In this work, we synthesized a selective and sensitive cationic fluorescent probe (ROPD) for monitoring exogenous and endogenous nitric oxide in live cells by grafting a NO-trapper o-phenylenediamine (OPD), via one of the amino groups of OPD, onto a rhodamine fluorophore at the C-3 position. The probe exhibited high selectivity and sensitivity towards NO with fast response in the water phase over a wide pH range. At the same time, the probe ROPD is cell-membrane permeable and can be used for the real-time visualization of exogenous NO in both L929 and HeLa cells, and endogenous NO in stressed RAW 264.7 cells.
一氧化氮是一种重要的细胞信使分子，在神经传递、血管舒张、血小板活化和免疫反应中发挥着广泛的生理作用。在这项工作中，我们通过将一种一氧化氮捕获剂邻苯二胺（OPD）经其一个氨基接枝到罗丹明荧光团的C-3位，合成了一种用于监测活细胞中外源性和内源性一氧化氮的选择性和灵敏的阳离子荧光探针（ROPD）。该探针在水相中、宽pH范围内对一氧化氮表现出高选择性和灵敏性以及快速响应。同时，探针ROPD具有细胞膜通透性，可用于实时可视化L929和HeLa细胞中的外源性一氧化氮以及应激RAW 264.7细胞中的内源性一氧化氮。
