Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine , Shanghai, China.
Beijing Tiantan Hospital, Capital MedicalUniversity , Beijing, China.
Curr Eye Res. 2020 Nov;45(11):1422-1429. doi: 10.1080/02713683.2020.1752738. Epub 2020 Apr 30.
: To evaluate whether optical imaging methods and histology can detect comparable vascular and neuronal damage in the retina due to the effects of progressive chronic hypertension on the retinal vasculature and neurons using the spontaneously hypertensive rat (SHR) model at young and old ages. : Male SHR and normotensive Wistar Kyoto (WKY) rats were studied at 10 and 40 weeks of age (n = 6 each group). Arterial blood pressure was measured with a tail-cuff. Under anesthesia, fundus photography was used to measure retinal arterial diameters and optical coherence tomography was used to measure retinal layer thicknesses. Histology was then used to measure microvascular and cell density in different retinal layers. : Blood pressure was significantly higher in SHR than WKY in both age groups ( < .05). Fundus images showed no gross abnormalities, hemorrhage, or stenosis in all groups. Retinal vessels, however, appeared more tortuous in SHR compared to WKY at both ages. Retinal vessel diameters in SHR were significantly narrower than in WKY at both age groups ( < .05). Microvascular densities at 10 weeks were not significantly different ( > .05) but were markedly reduced in SHR at 40 weeks compared to WKY ( < .05). The outer nuclear layer thickness of SHR was significantly thinner than that of WKY at both ages ( < .05), consistent with histological cell density measurements ( < .05). The ganglion cell layer and inner nuclear layer thicknesses were not significantly different between SHR and WKY ( > .05), consistent with the corresponding histological cell density measurements ( > .05). : optical imaging showed that systemic hypertension progressively reduces retinal arterial diameter and thicknesses of the outer retina in spontaneously hypertensive rats, with consistent vascular and neuronal findings from histology.
: 目的：利用自发性高血压大鼠（SHR）模型，评估光学成像方法和组织学是否能够检测到由于进行性慢性高血压对视网膜血管和神经元的影响而导致的视网膜血管和神经元的类似损伤。 : 雄性 SHR 和血压正常的 Wistar Kyoto（WKY）大鼠分别在 10 周和 40 周龄时进行研究（每组 n = 6）。采用尾套法测量动脉血压。在麻醉下，使用眼底照相术测量视网膜动脉直径，使用光学相干断层扫描术测量视网膜层厚度。然后使用组织学方法测量不同视网膜层中的微血管和细胞密度。 : 两组大鼠的 SHR 血压均显著高于 WKY（ < 0.05）。所有组的眼底图像均未显示明显的异常、出血或狭窄。然而，与 WKY 相比，在两个年龄段，SHR 的视网膜血管看起来更加扭曲。在两个年龄段，SHR 的视网膜血管直径均明显小于 WKY（ < 0.05）。10 周时，微血管密度无显著差异（ > 0.05），但 40 周时 SHR 明显低于 WKY（ < 0.05）。与 WKY 相比，在两个年龄段，SHR 的外核层厚度均明显较薄（ < 0.05），与组织学细胞密度测量结果一致（ < 0.05）。与 WKY 相比，SHR 的节细胞层和内核层厚度无显著差异（ > 0.05），与相应的组织学细胞密度测量结果一致（ > 0.05）。 : 光学成像显示，全身性高血压逐渐降低自发性高血压大鼠视网膜动脉直径和外视网膜厚度，与组织学的血管和神经元发现一致。
