Department of Experimental Zoology and Neurobiology, University of Pécs, 7624 Pécs, Hungary.
Department of Medical Biology, Medical School, University of Pécs, 7624 Pécs, Hungary.
Cells. 2021 Dec 9;10(12):3470. doi: 10.3390/cells10123470.
Diabetes and hypertension are complex pathologies with increasing prevalence nowadays. Their interconnected pathways are frequently manifested in retinopathies. Severe retinal consequences and their tight connections as well as their possible treatments are particularly important to retinal research. In the present, work we induced diabetes with streptozotocin in spontaneously hypertensive rats and treated them either with PACAP or olaparib and alternatively with both agents. Morphological and immunohistochemical analyses were carried out to describe cell-specific changes during pathologies and after different treatments. Diabetes and hypertension caused massive structural and cellular changes especially when they were elicited together. Hypertension was crucial in the formation of ONL and OPL damage while diabetes caused significant differences in retinal thickness, OPL thickness and in the cell number of the GCL. In diabetes, double neuroprotective treatment ameliorated changes of calbindin-positive cells, rod bipolar cells and dopaminergic amacrine cells. Double treatment was curative in hypertensive diabetic rat retinas, especially in the case of rod bipolar and parvalbumin-positive cells compared to untreated or single-treated retinas. Our results highlighted the promising therapeutic benefits of olaparib and PACAP in these severe metabolic retinal disorders.
糖尿病和高血压是现今患病率日益增加的复杂病理。它们相互关联的途径经常表现在视网膜病变中。严重的视网膜后果及其紧密联系以及它们可能的治疗方法对视网膜研究尤为重要。在目前的工作中，我们用链脲佐菌素诱导自发性高血压大鼠患上糖尿病，并分别用 PACAP 或奥拉帕利，或者两种药物交替治疗。进行形态学和免疫组织化学分析，以描述病理过程中和不同治疗后的细胞特异性变化。糖尿病和高血压引起了大量的结构和细胞变化，特别是当它们一起引发时。高血压对 ONL 和 OPL 损伤的形成至关重要，而糖尿病导致视网膜厚度、OPL 厚度和 GCL 细胞数量出现显著差异。在糖尿病中，双重神经保护治疗改善了 calbindin 阳性细胞、视杆双极细胞和多巴胺能无长突细胞的变化。与未治疗或单一治疗的视网膜相比，双重治疗对高血压糖尿病大鼠的视网膜具有治疗效果，尤其是在视杆双极细胞和 parvalbumin 阳性细胞方面。我们的结果强调了奥拉帕利和 PACAP 在这些严重代谢性视网膜疾病中的有前途的治疗益处。
